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- W2000906302 abstract "Physically and chemically stable microscopic aqueous droplets of nano-, pico-, and femtoliter volumes were made and kept under heptane. Such droplets can contain chemicals of interest in pico-, femto-, and attomole amounts or less. Their fine chemical manipulation was achieved by a diffusional microburet (DMB), which consists of a pulled glass capillary whose microscopic tip is filled with a tiny diffusion membrane of agar or polyacrylamide gel. Once this tip is moved into a target droplet by a fine micromanipulator under a microscope, diffusional reagent delivery from the DMB body begins, driven by the concentration gradient within the tip. This system was tested in this work by delivering an inert fluorescent dye, Lucifer Yellow CH, into buffer droplets while fluorescence intensity in each droplet was recorded. An exponential decay of delivery rate was observed corresponding to an exponential saturation process for the accumulation of the delivered chemical inside the target droplet." @default.
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- W2000906302 date "1999-06-10" @default.
- W2000906302 modified "2023-10-16" @default.
- W2000906302 title "Fine Chemical Manipulations of Microscopic Liquid Samples. 1. Droplet Loading with Chemicals" @default.
- W2000906302 cites W1987757832 @default.
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- W2000906302 doi "https://doi.org/10.1021/ac980929q" @default.
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