Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000929543> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2000929543 endingPage "219" @default.
- W2000929543 startingPage "203" @default.
- W2000929543 abstract "Purified human haptoglobins have been subjected to reductive cleavage by β-mercaptoethanol in 8 M urea or 6 M guanidine. The reduction of haptoglobin 1-1 and 2-2 exposes 18 and 24 SH groups/mole of protein, respectively. Removal of denaturing reagents and reactivation in the presence of O2 and a catalytic amount of thiol was followed by the regain of the chemical and immunological properties of the native protein. Under the most favorable conditions of reoxidation the yield of haptoglobin, estimated by molecular size and hemoglobin binding capacity, ranged between 60 and 90%. Reactivation was also obtained when equimolecular mixtures of isolated and separately reactivated α and β chains were incubated at pH 8.15 and 20°. The formation of a stable complex between β-haptoglobin and an hemoglobin dimer (αβ) did not interfere with the reassociation of the former with the α1 haptoglobin chain. The refolding process is highly specific and occurs (although with a lower yield of protein) also when haptoglobins are reduced and reoxidized together with other proteins containing SH groups." @default.
- W2000929543 created "2016-06-24" @default.
- W2000929543 creator A5007857377 @default.
- W2000929543 creator A5074019502 @default.
- W2000929543 date "1970-02-01" @default.
- W2000929543 modified "2023-10-13" @default.
- W2000929543 title "Studies on the structure of human haptoglobins I. Spontaneous refolding after extensive reduction and dissociation" @default.
- W2000929543 cites W145568566 @default.
- W2000929543 cites W1485449426 @default.
- W2000929543 cites W1504241430 @default.
- W2000929543 cites W1586757195 @default.
- W2000929543 cites W1602712226 @default.
- W2000929543 cites W1980293817 @default.
- W2000929543 cites W2000182699 @default.
- W2000929543 cites W2006370393 @default.
- W2000929543 cites W2007436249 @default.
- W2000929543 cites W2019628741 @default.
- W2000929543 cites W2032075847 @default.
- W2000929543 cites W2041113419 @default.
- W2000929543 cites W2043446894 @default.
- W2000929543 cites W2046547763 @default.
- W2000929543 cites W2059314799 @default.
- W2000929543 cites W2081917360 @default.
- W2000929543 cites W2087664001 @default.
- W2000929543 cites W2401807178 @default.
- W2000929543 doi "https://doi.org/10.1016/0005-2795(70)90165-0" @default.
- W2000929543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4984555" @default.
- W2000929543 hasPublicationYear "1970" @default.
- W2000929543 type Work @default.
- W2000929543 sameAs 2000929543 @default.
- W2000929543 citedByCount "24" @default.
- W2000929543 crossrefType "journal-article" @default.
- W2000929543 hasAuthorship W2000929543A5007857377 @default.
- W2000929543 hasAuthorship W2000929543A5074019502 @default.
- W2000929543 hasConcept C102931765 @default.
- W2000929543 hasConcept C134121241 @default.
- W2000929543 hasConcept C178790620 @default.
- W2000929543 hasConcept C185592680 @default.
- W2000929543 hasConcept C191897082 @default.
- W2000929543 hasConcept C192562407 @default.
- W2000929543 hasConcept C203014093 @default.
- W2000929543 hasConcept C206396517 @default.
- W2000929543 hasConcept C2778917026 @default.
- W2000929543 hasConcept C2779463914 @default.
- W2000929543 hasConcept C2779546866 @default.
- W2000929543 hasConcept C2780365088 @default.
- W2000929543 hasConcept C2781324293 @default.
- W2000929543 hasConcept C40875361 @default.
- W2000929543 hasConcept C43617362 @default.
- W2000929543 hasConcept C55493867 @default.
- W2000929543 hasConcept C86803240 @default.
- W2000929543 hasConceptScore W2000929543C102931765 @default.
- W2000929543 hasConceptScore W2000929543C134121241 @default.
- W2000929543 hasConceptScore W2000929543C178790620 @default.
- W2000929543 hasConceptScore W2000929543C185592680 @default.
- W2000929543 hasConceptScore W2000929543C191897082 @default.
- W2000929543 hasConceptScore W2000929543C192562407 @default.
- W2000929543 hasConceptScore W2000929543C203014093 @default.
- W2000929543 hasConceptScore W2000929543C206396517 @default.
- W2000929543 hasConceptScore W2000929543C2778917026 @default.
- W2000929543 hasConceptScore W2000929543C2779463914 @default.
- W2000929543 hasConceptScore W2000929543C2779546866 @default.
- W2000929543 hasConceptScore W2000929543C2780365088 @default.
- W2000929543 hasConceptScore W2000929543C2781324293 @default.
- W2000929543 hasConceptScore W2000929543C40875361 @default.
- W2000929543 hasConceptScore W2000929543C43617362 @default.
- W2000929543 hasConceptScore W2000929543C55493867 @default.
- W2000929543 hasConceptScore W2000929543C86803240 @default.
- W2000929543 hasIssue "2" @default.
- W2000929543 hasLocation W20009295431 @default.
- W2000929543 hasLocation W20009295432 @default.
- W2000929543 hasOpenAccess W2000929543 @default.
- W2000929543 hasPrimaryLocation W20009295431 @default.
- W2000929543 hasRelatedWork W2037010243 @default.
- W2000929543 hasRelatedWork W2039645710 @default.
- W2000929543 hasRelatedWork W2043343265 @default.
- W2000929543 hasRelatedWork W2044217213 @default.
- W2000929543 hasRelatedWork W2053341833 @default.
- W2000929543 hasRelatedWork W2131945995 @default.
- W2000929543 hasRelatedWork W2419373478 @default.
- W2000929543 hasRelatedWork W2420075412 @default.
- W2000929543 hasRelatedWork W2473403504 @default.
- W2000929543 hasRelatedWork W4285008569 @default.
- W2000929543 hasVolume "200" @default.
- W2000929543 isParatext "false" @default.
- W2000929543 isRetracted "false" @default.
- W2000929543 magId "2000929543" @default.
- W2000929543 workType "article" @default.