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• W2000934444 abstract "The aim of this study was to evaluate and compare the effects of two commercial mineral trioxide aggregate (MTA) cements (ProRoot MTA and MTA Angelus) on transforming growth factor (TGF)-β1 and bone morphogenetic protein (BMP)-2 levels produced by cultured human gingival fibroblasts (HGFs). Human gingival tissues were obtained from individuals with healthy periodontium. HGFs were grown at 37°C in humidified atmosphere of 5% CO2 in Dulbecco’s modified Eagle’s medium, supplemented with 10% fetal calf serum, penicillin, and streptomycin. After 24 and 72 hours of exposure to the MTA products, HGF viability was determined by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay. TGF-β1 and BMP-2 levels in cell-free culture media were determined by enzyme-linked immunosorbent assay. Cell viability of the test groups was significantly lower than that of control at 24 and 72 hours (p < 0.05) but showed an increase at 72 hours (p < 0.05). Both test groups showed increased TGF β-1 levels at 72 hours (p < 0.05), whereas the MTA Angelus group displayed higher TGF β-1 levels than control and ProRoot MTA groups at 24 and 72 hours (p < 0.05). At 24 hours, BMP-2 levels of the ProRoot group were significantly higher than that of MTA Angelus (p < 0.05). Both test materials increased the BMP-2 levels within time (p < 0.05) and displayed similar levels at 72 hours (p > 0.05). These results suggest that both MTA products are capable of stimulating HGF to produce BMP-2, whereas the stimulatory effect for TGF β-1 is material dependent." @default.
• W2000934444 created "2016-06-24" @default.
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• W2000934444 date "2007-04-01" @default.
• W2000934444 modified "2023-10-18" @default.
• W2000934444 title "Effect of Mineral Trioxide Aggregate Cements on Transforming Growth Factor β1 and Bone Morphogenetic Protein Production by Human Fibroblasts In Vitro" @default.
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• W2000934444 doi "https://doi.org/10.1016/j.joen.2006.12.020" @default.
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