Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000936044> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2000936044 endingPage "122" @default.
- W2000936044 startingPage "113" @default.
- W2000936044 abstract "The conditions previously reported as necessary for the reassembly of spherical viruses have been distinctly unphysiological and such reassembly cannot be related directly to the in vivo reaction. Mild conditions for the in vitro reassembly of cowpea chlorotic mottle virus (CCMV) from its isolated components have now been described (Adolph & Butler 1975) and the reassembled virus characterized. This reassembly involved the co-aggregation of the RNA and protein around neutrality and at ionic strength 0.2, giving yields of 70% encapsidation at pH 6.0. The reaction was independent of temperature over the range 5-25 degrees C and did not require the presence of Mg2+ ions. The reassembled virus shows a stability similar to that of native CCMV, with the same change in sedimentation coefficient around pH 6.5. The molecular mass and buoyant density in CsCl are also the same as those of native CCMV, while the electron microscope reveals a surface morphology on the reassembled particles like that on native CCMV. Analysis of the number-average, mass-average, and Z-average molecular masses of the purified protein at both pH 6.0 and pH 7.5 suggests that the active unit for reassembly is a dimer of the protein subunit." @default.
- W2000936044 created "2016-06-24" @default.
- W2000936044 creator A5032124280 @default.
- W2000936044 creator A5032364292 @default.
- W2000936044 date "1976-11-30" @default.
- W2000936044 modified "2023-09-25" @default.
- W2000936044 title "Assembly of a spherical plant virus" @default.
- W2000936044 doi "https://doi.org/10.1098/rstb.1976.0102" @default.
- W2000936044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/13422" @default.
- W2000936044 hasPublicationYear "1976" @default.
- W2000936044 type Work @default.
- W2000936044 sameAs 2000936044 @default.
- W2000936044 citedByCount "39" @default.
- W2000936044 countsByYear W20009360442012 @default.
- W2000936044 countsByYear W20009360442013 @default.
- W2000936044 countsByYear W20009360442014 @default.
- W2000936044 countsByYear W20009360442015 @default.
- W2000936044 countsByYear W20009360442016 @default.
- W2000936044 countsByYear W20009360442017 @default.
- W2000936044 countsByYear W20009360442019 @default.
- W2000936044 countsByYear W20009360442020 @default.
- W2000936044 countsByYear W20009360442021 @default.
- W2000936044 countsByYear W20009360442023 @default.
- W2000936044 crossrefType "journal-article" @default.
- W2000936044 hasAuthorship W2000936044A5032124280 @default.
- W2000936044 hasAuthorship W2000936044A5032364292 @default.
- W2000936044 hasConcept C104292427 @default.
- W2000936044 hasConcept C104317684 @default.
- W2000936044 hasConcept C120665830 @default.
- W2000936044 hasConcept C121332964 @default.
- W2000936044 hasConcept C12554922 @default.
- W2000936044 hasConcept C1388644 @default.
- W2000936044 hasConcept C147789679 @default.
- W2000936044 hasConcept C159047783 @default.
- W2000936044 hasConcept C178790620 @default.
- W2000936044 hasConcept C181199279 @default.
- W2000936044 hasConcept C184651966 @default.
- W2000936044 hasConcept C185592680 @default.
- W2000936044 hasConcept C187428577 @default.
- W2000936044 hasConcept C2522874641 @default.
- W2000936044 hasConcept C2779546866 @default.
- W2000936044 hasConcept C55493867 @default.
- W2000936044 hasConcept C58121356 @default.
- W2000936044 hasConcept C8010536 @default.
- W2000936044 hasConcept C86803240 @default.
- W2000936044 hasConcept C93877712 @default.
- W2000936044 hasConceptScore W2000936044C104292427 @default.
- W2000936044 hasConceptScore W2000936044C104317684 @default.
- W2000936044 hasConceptScore W2000936044C120665830 @default.
- W2000936044 hasConceptScore W2000936044C121332964 @default.
- W2000936044 hasConceptScore W2000936044C12554922 @default.
- W2000936044 hasConceptScore W2000936044C1388644 @default.
- W2000936044 hasConceptScore W2000936044C147789679 @default.
- W2000936044 hasConceptScore W2000936044C159047783 @default.
- W2000936044 hasConceptScore W2000936044C178790620 @default.
- W2000936044 hasConceptScore W2000936044C181199279 @default.
- W2000936044 hasConceptScore W2000936044C184651966 @default.
- W2000936044 hasConceptScore W2000936044C185592680 @default.
- W2000936044 hasConceptScore W2000936044C187428577 @default.
- W2000936044 hasConceptScore W2000936044C2522874641 @default.
- W2000936044 hasConceptScore W2000936044C2779546866 @default.
- W2000936044 hasConceptScore W2000936044C55493867 @default.
- W2000936044 hasConceptScore W2000936044C58121356 @default.
- W2000936044 hasConceptScore W2000936044C8010536 @default.
- W2000936044 hasConceptScore W2000936044C86803240 @default.
- W2000936044 hasConceptScore W2000936044C93877712 @default.
- W2000936044 hasIssue "943" @default.
- W2000936044 hasLocation W20009360441 @default.
- W2000936044 hasLocation W20009360442 @default.
- W2000936044 hasOpenAccess W2000936044 @default.
- W2000936044 hasPrimaryLocation W20009360441 @default.
- W2000936044 hasRelatedWork W1965202681 @default.
- W2000936044 hasRelatedWork W1965701922 @default.
- W2000936044 hasRelatedWork W1972220625 @default.
- W2000936044 hasRelatedWork W1985665467 @default.
- W2000936044 hasRelatedWork W2005565524 @default.
- W2000936044 hasRelatedWork W2007527061 @default.
- W2000936044 hasRelatedWork W2011450357 @default.
- W2000936044 hasRelatedWork W2028338572 @default.
- W2000936044 hasRelatedWork W2152588655 @default.
- W2000936044 hasRelatedWork W2411173291 @default.
- W2000936044 hasVolume "276" @default.
- W2000936044 isParatext "false" @default.
- W2000936044 isRetracted "false" @default.
- W2000936044 magId "2000936044" @default.
- W2000936044 workType "article" @default.