FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000937010> ?p ?o ?g. }

• W2000937010 endingPage "201" @default.
• W2000937010 startingPage "191" @default.
• W2000937010 abstract "Lysosomotropic animo acid esters and amides kill Leishmania amazonensis amastigotes by a mechanism which probably involves enzymatic hydrolysis of the compounds and rapid accumulation of less permeant amino acid within the parasites. We show here that, in agreement with this model, the proteinase inhibitors antipain and chymostatin prevented the killing of intracellular and isolated parasites by l-leucine methyl ester (Leu-OMe). Survival of Leishmania within macrophages was assessed microscopically, and that of isolated amastigotes was measured by tetrazolium (MTT) reduction. Near maximal protection of intracellular parasites was obtained after 24 h incubation of macrophage cultures with 50 μg ml−1 of antipain or chymostatin. In contrast, isolated amastigotes were significantly protected after 15–60 min incubation with as little as 0.25 μg ml−1 antipain or chymostatin. Incubation for > 1 h with chymostatin or > 4 h with antipain alone resulted in loss of viability of the parasites. Protective activity was only slightly diminished by 20 h chase of isolated parasites in inhibitor-free medium. Two synthetic chymostatin analogues, Z-Val-Phe-Se and Z-Ile-Phe-Sc, protected isolated amastigotes at 4 or 10 μg ml−1. With the exception of Trp-NH2, the toxicity of which was only minimally inhibited, antipain and chymostatin also prevented parasite destruction by other amino acid derivatives. Finally, in concentration-dependent fashion, the inhibitors reduced the accumulation of [3H]leucine in isolated amastigotes incubated with [3H]Leu-OMe. Since uptake of labelled ester was unaffected, we postulate that protection involves inhibition of the parasite enzymes which hydrolyse the amino acid derivatives." @default.
• W2000937010 created "2016-06-24" @default.
• W2000937010 creator A5001074970 @default.
• W2000937010 creator A5028944490 @default.
• W2000937010 creator A5032399396 @default.
• W2000937010 creator A5033177538 @default.
• W2000937010 creator A5052047759 @default.
• W2000937010 creator A5053657648 @default.
• W2000937010 date "1988-06-01" @default.
• W2000937010 modified "2023-09-25" @default.
• W2000937010 title "Proteinase inhibitors protect Leishmania amazonensis amastigotes from destruction by amino acid esters" @default.
• W2000937010 cites W1160821720 @default.
• W2000937010 cites W1537836578 @default.
• W2000937010 cites W1577607250 @default.
• W2000937010 cites W1577785183 @default.
• W2000937010 cites W1606963850 @default.
• W2000937010 cites W1973111776 @default.
• W2000937010 cites W1981459156 @default.
• W2000937010 cites W1986524729 @default.
• W2000937010 cites W2001590903 @default.
• W2000937010 cites W2016226091 @default.
• W2000937010 cites W2044084748 @default.
• W2000937010 cites W2061974162 @default.
• W2000937010 cites W2088942144 @default.
• W2000937010 cites W2110893184 @default.
• W2000937010 cites W2114918609 @default.
• W2000937010 cites W2128913117 @default.
• W2000937010 cites W2130303623 @default.
• W2000937010 cites W2140927361 @default.
• W2000937010 cites W2149566210 @default.
• W2000937010 cites W2163621688 @default.
• W2000937010 cites W2173786024 @default.
• W2000937010 cites W2410774316 @default.
• W2000937010 cites W2429334101 @default.
• W2000937010 cites W250739818 @default.
• W2000937010 doi "https://doi.org/10.1016/0166-6851(88)90074-6" @default.
• W2000937010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3412375" @default.
• W2000937010 hasPublicationYear "1988" @default.
• W2000937010 type Work @default.
• W2000937010 sameAs 2000937010 @default.
• W2000937010 citedByCount "19" @default.
• W2000937010 countsByYear W20009370102019 @default.
• W2000937010 crossrefType "journal-article" @default.
• W2000937010 hasAuthorship W2000937010A5001074970 @default.
• W2000937010 hasAuthorship W2000937010A5028944490 @default.
• W2000937010 hasAuthorship W2000937010A5032399396 @default.
• W2000937010 hasAuthorship W2000937010A5033177538 @default.
• W2000937010 hasAuthorship W2000937010A5052047759 @default.
• W2000937010 hasAuthorship W2000937010A5053657648 @default.
• W2000937010 hasConcept C136764020 @default.
• W2000937010 hasConcept C153911025 @default.
• W2000937010 hasConcept C181199279 @default.
• W2000937010 hasConcept C202751555 @default.
• W2000937010 hasConcept C25642318 @default.
• W2000937010 hasConcept C2775883766 @default.
• W2000937010 hasConcept C2776350231 @default.
• W2000937010 hasConcept C2776580952 @default.
• W2000937010 hasConcept C2776714187 @default.
• W2000937010 hasConcept C2777752497 @default.
• W2000937010 hasConcept C2781092759 @default.
• W2000937010 hasConcept C2781378782 @default.
• W2000937010 hasConcept C41008148 @default.
• W2000937010 hasConcept C515207424 @default.
• W2000937010 hasConcept C55493867 @default.
• W2000937010 hasConcept C71928629 @default.
• W2000937010 hasConcept C79879829 @default.
• W2000937010 hasConcept C86803240 @default.
• W2000937010 hasConcept C98319499 @default.
• W2000937010 hasConceptScore W2000937010C136764020 @default.
• W2000937010 hasConceptScore W2000937010C153911025 @default.
• W2000937010 hasConceptScore W2000937010C181199279 @default.
• W2000937010 hasConceptScore W2000937010C202751555 @default.
• W2000937010 hasConceptScore W2000937010C25642318 @default.
• W2000937010 hasConceptScore W2000937010C2775883766 @default.
• W2000937010 hasConceptScore W2000937010C2776350231 @default.
• W2000937010 hasConceptScore W2000937010C2776580952 @default.
• W2000937010 hasConceptScore W2000937010C2776714187 @default.
• W2000937010 hasConceptScore W2000937010C2777752497 @default.
• W2000937010 hasConceptScore W2000937010C2781092759 @default.
• W2000937010 hasConceptScore W2000937010C2781378782 @default.
• W2000937010 hasConceptScore W2000937010C41008148 @default.
• W2000937010 hasConceptScore W2000937010C515207424 @default.
• W2000937010 hasConceptScore W2000937010C55493867 @default.
• W2000937010 hasConceptScore W2000937010C71928629 @default.
• W2000937010 hasConceptScore W2000937010C79879829 @default.
• W2000937010 hasConceptScore W2000937010C86803240 @default.
• W2000937010 hasConceptScore W2000937010C98319499 @default.
• W2000937010 hasIssue "2-3" @default.
• W2000937010 hasLocation W20009370101 @default.
• W2000937010 hasLocation W20009370102 @default.
• W2000937010 hasOpenAccess W2000937010 @default.
• W2000937010 hasPrimaryLocation W20009370101 @default.
• W2000937010 hasRelatedWork W1931085048 @default.
• W2000937010 hasRelatedWork W1980802011 @default.
• W2000937010 hasRelatedWork W1984956164 @default.
• W2000937010 hasRelatedWork W1992951975 @default.
• W2000937010 hasRelatedWork W1997024873 @default.
• W2000937010 hasRelatedWork W2000937010 @default.

• next