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• W2000937674 startingPage "54" @default.
• W2000937674 abstract "Metallothioneins (MT) are a validated biomarker for metal exposure. In fish they are usually determined in the liver, while some studies have observed MT in kidney, muscle and gills. There are, however, no reports on MT in fish bile. This study aimed to verify if these proteins are present in fish bile, if they can be used as biomarkers for metal exposure and to characterize the metal speciation present in the different species. Three independent methods to verify MT presence in fish bile were conducted: 1D/2D gel electrophoresis, spectrophotometric analyses and SEC-HPLC-ICP-MS. Results show that all methods verified the presence of these proteins in fish bile, while SEC-HPLC-ICP-MS also characterized also characterized metals responsible for the contamination of the studied sites. Quantification was successfully conducted by two methods, densitometry (1D electrophoresis) and spectrophotometry, and the data indicates that bile MTs follow the same trend as in liver. Therefore, Biliary MT show the potential to be used the same way as MT from other matrices in metal exposure studies, with the advantage of being a simpler matrix and showing the possibility of not sacrificing the animals, as chronic duct cannulation is available." @default.
• W2000937674 created "2016-06-24" @default.
• W2000937674 creator A5003773176 @default.
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• W2000937674 date "2012-07-01" @default.
• W2000937674 modified "2023-09-28" @default.
• W2000937674 title "A novel report of metallothioneins in fish bile: SDS-PAGE analysis, spectrophotometry quantification and metal speciation characterization by liquid chromatography coupled to ICP-MS" @default.
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• W2000937674 doi "https://doi.org/10.1016/j.aquatox.2012.03.003" @default.
• W2000937674 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22466355" @default.
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