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- W2000938558 abstract "The DNA's of the two small Bacillus subtilis phages, ∅29 and ∅15, were analyzed by use of two restriction endonucleases, EcoR1 and HpaI. Five and six EcoR1 generated fragments were obtained from ∅29 and ∅15 genomes, respectively. Eight HpaI generated fragments were obtained from each phage genome, although the sizes of the fragments were considerably different. The molecular weights of each fragment were estimated from the distribution of 32P-radioactivity in the bands and from the relative electrophoretic mobilities. The five fragments of ∅29 DNA by EcoR1 were isolated in pure form and their sedimentation rates were determined by sucrose gradient centrifugation. The four EcoR1 restriction sites in ∅29 DNA have been mapped by analysis of incomplete digestion products. The ends of the DNA molecules were identified by analysis of EcoR1 digestion fragments of nonprotease-treated ∅29 DNA using agarose gel electrophoresis. From similar experiments, the order of the six fragments of ∅15 DNA produced by EcoR1 digestion is also proposed." @default.
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- W2000938558 date "1976-03-01" @default.
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- W2000938558 title "Analysis of ∅29 and ∅15 genomes by bacterial restriction endonucleases, EcoR1 and HpaI" @default.
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- W2000938558 doi "https://doi.org/10.1016/0042-6822(76)90234-8" @default.
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