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- W2000942140 abstract "Conditions for isolating intact and active nuclei from human term placenta and for studying their transcription products are described. The isolated nuclei can synthesize cell-free RNA for a prolonged period at 29°C in a medium containing 100 mm KCl and 5 mm MgCl2. Actinomycin D inhibited 92 per cent of RNA synthesis, whereas approximately 60 per cent of RNA synthesis was sensitive to α-amanitin. When nuclei were incubated at 29°C for 1 h, about 27 per cent of the newly synthesized RNA was released into the medium outside the nucleus. Analysis of this released material by affinity chromatography on an oligo(dT)-cellulose column revealed that 2.4 per cent of the total released RNA was adsorbed at high salt concentration. Most of this fraction was eluted with a low-salt buffer at 45°C and the remainder by 50 per cent formamide, conditions that are necessary for elution of poly(A)-containing mRNP particles from oligo(dT)-cellulose. These results show that placental nuclei incubated in vitro synthesize poly(A)-containing RNA, which is released as a protein-bound complex. This procedure allows exploration of changes in mRNA release during placental development." @default.
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- W2000942140 date "1980-04-01" @default.
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- W2000942140 title "Synthesis and processing of RNA by isolated human placental nuclei" @default.
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- W2000942140 doi "https://doi.org/10.1016/s0143-4004(80)80025-7" @default.
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