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- W2000952941 abstract "Two techniques were investigated to find a laboratory method for the prediction of the in situ nitrogen degradation of concentrated feeds: a method using solubility in a buffer (phosphate buffer at pH 6.9) and an enzymatic technique (hydrolysis after 1 h (DE1) and after 24 h (DE24) by a protease extracted from Streptomyces griseus in a borate-phosphate buffer at pH 8). Ninety-seven samples of concentrated feeds, representing 26 single feeds and 49 commercial feed mixtures, were studied. Both methods allowed the same precision (residual standard deviation (RSD) ∼ 0.030) if the 26 single feeds were grouped in classes. In contrast, for feed mixtures, the prediction was very precise and much better with the enzymatic method (RSD = 0.025) than with the solubility method (RSD = 0.049). In the French digestible proteins in the intestine (PDI) system, the enzymatic method is used as a laboratory method for nitrogen evaluation." @default.
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- W2000952941 date "1991-04-01" @default.
- W2000952941 modified "2023-10-18" @default.
- W2000952941 title "Predicting in situ degradability of feed proteins in the rumen by two laboratory methods (solubility and enzymatic degradation)" @default.
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- W2000952941 doi "https://doi.org/10.1016/0377-8401(91)90049-x" @default.
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