Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000964053> ?p ?o ?g. }
- W2000964053 endingPage "686" @default.
- W2000964053 startingPage "669" @default.
- W2000964053 abstract "1. The effects of activation of GABAB receptors on Ca2+ currents (ICa) were investigated by application of whole-cell patch-clamp techniques to pyramidal neurones and non-pyramidal interneurones from the rat hippocampus grown in cell culture. 2. (+/-)-Baclofen (10 microM) reduced ICa evoked in pyramidal neurones at 0 mV from a holding potential of -80 mV by 33 +/- 3%. Inhibition could be observed at the peak of ICa with significant inhibition still present after 200 ms at 0 mV. When Ba2+ was used as the charge carrier (IBa) baclofen inhibited 28 +/- 3% of the current at -20 mV from a holding potential of -80 mV. The GABAB receptor antagonist 2-OH-saclofen (50-200 microM) blocked the actions of baclofen. 3. The selective Ca2+ channel blocker, omega-conotoxin fraction GVIA (omega-CgTX), was used to characterize the Ca2+ currents inhibited by baclofen. omega-CgTX (5 microM) blocked 24 +/- 3% of IBa. Following block of the omega-CgTX-sensitive current, baclofen inhibited significantly less current than under control conditions. 4. Addition of the dihydropyridine Ca2+ channel antagonist nimodipine (1 microM) inhibited 18 +/- 5% of ICa at 0 mV from a holding potential of -80 mV and 44 +/- 9% from a holding potential of -40 mV. In addition, nimodipine partially occluded subsequent responses to application of baclofen. 5. In the presence of both 5 microM-omega-CgTX and 200 nM-nimodipine, responses to baclofen were almost completely blocked at depolarized holding potentials where the dihydropyridines are most effective. 6. Inclusion of 500 microM-guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) in the patch pipette enhanced the response to a subsaturating concentration of baclofen and rendered the response irreversible. Subsequent addition of the adenosine receptor agonist 2-Cl-adenosine (2-CA) (1 microM; which also reduces ICa under control conditions) was without effect, suggesting that these two receptor-effector pathways converge. 7. The actions of baclofen on ICa were blocked by pre-treatment of the cultures with pertussis toxin (250 ng/ml). 8. Baclofen also inhibited ICa in non-pyramidal neurones from the hippocampus, but was slightly less effective. 9. Baclofen reduced both excitatory- and inhibitory postsynaptic currents (EPSCs and IPSCs) recorded as a consequence of extracellular stimulation of presynaptic neurones. This action was blocked by 2-OH-saclofen (200 microM) and also by pretreatment of the cultures with pertussis toxin.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
- W2000964053 created "2016-06-24" @default.
- W2000964053 creator A5061375376 @default.
- W2000964053 creator A5066616314 @default.
- W2000964053 date "1991-12-01" @default.
- W2000964053 modified "2023-10-08" @default.
- W2000964053 title "GABAB receptor-mediated inhibition of Ca2+ currents and synaptic transmission in cultured rat hippocampal neurones." @default.
- W2000964053 cites W1524359382 @default.
- W2000964053 cites W1582690177 @default.
- W2000964053 cites W1762877386 @default.
- W2000964053 cites W1866333058 @default.
- W2000964053 cites W1895927013 @default.
- W2000964053 cites W1975018922 @default.
- W2000964053 cites W1983423336 @default.
- W2000964053 cites W1985087389 @default.
- W2000964053 cites W1986009923 @default.
- W2000964053 cites W1987697310 @default.
- W2000964053 cites W1992682983 @default.
- W2000964053 cites W1993940179 @default.
- W2000964053 cites W1994090626 @default.
- W2000964053 cites W1994917583 @default.
- W2000964053 cites W1997511216 @default.
- W2000964053 cites W1997715717 @default.
- W2000964053 cites W2000552368 @default.
- W2000964053 cites W2009667219 @default.
- W2000964053 cites W2011138141 @default.
- W2000964053 cites W2015426350 @default.
- W2000964053 cites W2018931883 @default.
- W2000964053 cites W2029321368 @default.
- W2000964053 cites W2030740718 @default.
- W2000964053 cites W2034038906 @default.
- W2000964053 cites W2035282305 @default.
- W2000964053 cites W2039231750 @default.
- W2000964053 cites W2040021474 @default.
- W2000964053 cites W2044051019 @default.
- W2000964053 cites W2049230063 @default.
- W2000964053 cites W2055012870 @default.
- W2000964053 cites W2059919836 @default.
- W2000964053 cites W2064748241 @default.
- W2000964053 cites W2065959431 @default.
- W2000964053 cites W2068116440 @default.
- W2000964053 cites W2068617048 @default.
- W2000964053 cites W2071230662 @default.
- W2000964053 cites W2076095787 @default.
- W2000964053 cites W2077091381 @default.
- W2000964053 cites W2078316320 @default.
- W2000964053 cites W2080418204 @default.
- W2000964053 cites W2093428018 @default.
- W2000964053 cites W2093678094 @default.
- W2000964053 cites W2098070567 @default.
- W2000964053 cites W2119841109 @default.
- W2000964053 cites W2132198678 @default.
- W2000964053 cites W2149372271 @default.
- W2000964053 cites W2180452219 @default.
- W2000964053 cites W2404767277 @default.
- W2000964053 doi "https://doi.org/10.1113/jphysiol.1991.sp018900" @default.
- W2000964053 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1179955" @default.
- W2000964053 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1668352" @default.
- W2000964053 hasPublicationYear "1991" @default.
- W2000964053 type Work @default.
- W2000964053 sameAs 2000964053 @default.
- W2000964053 citedByCount "174" @default.
- W2000964053 countsByYear W20009640532012 @default.
- W2000964053 countsByYear W20009640532013 @default.
- W2000964053 countsByYear W20009640532015 @default.
- W2000964053 countsByYear W20009640532016 @default.
- W2000964053 countsByYear W20009640532017 @default.
- W2000964053 countsByYear W20009640532018 @default.
- W2000964053 countsByYear W20009640532020 @default.
- W2000964053 countsByYear W20009640532021 @default.
- W2000964053 countsByYear W20009640532022 @default.
- W2000964053 crossrefType "journal-article" @default.
- W2000964053 hasAuthorship W2000964053A5061375376 @default.
- W2000964053 hasAuthorship W2000964053A5066616314 @default.
- W2000964053 hasBestOaLocation W20009640531 @default.
- W2000964053 hasConcept C101027131 @default.
- W2000964053 hasConcept C12554922 @default.
- W2000964053 hasConcept C147944092 @default.
- W2000964053 hasConcept C148762608 @default.
- W2000964053 hasConcept C153461711 @default.
- W2000964053 hasConcept C169760540 @default.
- W2000964053 hasConcept C170493617 @default.
- W2000964053 hasConcept C178790620 @default.
- W2000964053 hasConcept C185592680 @default.
- W2000964053 hasConcept C18699975 @default.
- W2000964053 hasConcept C200170125 @default.
- W2000964053 hasConcept C2776885963 @default.
- W2000964053 hasConcept C2777294900 @default.
- W2000964053 hasConcept C2778071365 @default.
- W2000964053 hasConcept C2778938600 @default.
- W2000964053 hasConcept C2781201050 @default.
- W2000964053 hasConcept C2781212610 @default.
- W2000964053 hasConcept C519063684 @default.
- W2000964053 hasConcept C55493867 @default.
- W2000964053 hasConcept C71924100 @default.
- W2000964053 hasConcept C85520022 @default.
- W2000964053 hasConcept C86803240 @default.
- W2000964053 hasConcept C98274493 @default.