FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000966830> ?p ?o ?g. }

• W2000966830 endingPage "16538" @default.
• W2000966830 startingPage "16529" @default.
• W2000966830 abstract "The mechanism of inhibition of porcine leukocyte 12-lipoxygenase by 4-(2-oxapentadeca-4-yne)phenylpropanoic acid (OPP) was investigated. This compound is selective for the leukocyte form of the 12-lipoxygenase and inhibits the purified recombinant enzyme with an IC(50) value of approximately 2 microM. OPP induced a concentration-dependent lag phase in the oxygenation of arachidonic acid and decreased the maximal rate of reaction. Addition of the fatty acid hydroperoxide 13(S)-hydroperoxyoctadecadienoic acid (13-HPODE) to the reaction greatly reduced the OPP-induced lag. Lineweaver-Burk analysis of the effect of OPP on 12-lipoxygenase kinetics with arachidonic acid indicated that it was a mixed-type inhibitor. OPP was not metabolized by 12-lipoxygenase as evidenced by its quantitative recovery from incubations with stoichiometric amounts of enzyme and 13-HPODE or arachidonic acid. OPP inhibited the pseudoperoxidase activity of the enzyme with 13-HPODE and the reducing agent, BWA137C. Lineweaver-Burk analysis of the effect of OPP on pseudoperoxidase kinetics suggested that OPP was competitive with 13-HPODE. Single-turnover experiments indicated that OPP inhibited the reduction of 13-HPODE by a stoichiometric amount of ferrous 12-lipoxygenase. Addition of 13-HPODE shortened the OPP-induced lag phase but did not affect the maximal rate of enzyme activity. In addition, OPP had no effect on total product formation in either the presence or the absence of 5 microM 13-HPODE when the reaction was allowed to go to completion. All of these observations are consistent with a model for inhibition of 12-lipoxygenase activity in which OPP slows the oxidation of the inactive ferrous enzyme to the active ferric enzyme and competes with arachidonic acid for the ferric enzyme." @default.
• W2000966830 created "2016-06-24" @default.
• W2000966830 creator A5032158458 @default.
• W2000966830 creator A5038479973 @default.
• W2000966830 creator A5089031929 @default.
• W2000966830 date "1999-11-18" @default.
• W2000966830 modified "2023-10-16" @default.
• W2000966830 title "Mechanism of Inhibition of Porcine Leukocyte 12-Lipoxygenase by the Isoform-Specific Inhibitor 4-(2-Oxapentadeca-4-yne)phenylpropanoic Acid" @default.
• W2000966830 cites W1511636861 @default.
• W2000966830 cites W1622983148 @default.
• W2000966830 cites W1992794298 @default.
• W2000966830 cites W1999745429 @default.
• W2000966830 cites W2000864401 @default.
• W2000966830 cites W2013592393 @default.
• W2000966830 cites W2048619099 @default.
• W2000966830 cites W2064374001 @default.
• W2000966830 cites W2068946779 @default.
• W2000966830 cites W2073617742 @default.
• W2000966830 cites W2077966190 @default.
• W2000966830 cites W2106153387 @default.
• W2000966830 cites W2125720771 @default.
• W2000966830 cites W2340902442 @default.
• W2000966830 cites W2413658395 @default.
• W2000966830 cites W2949340387 @default.
• W2000966830 doi "https://doi.org/10.1021/bi991336s" @default.
• W2000966830 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10600114" @default.
• W2000966830 hasPublicationYear "1999" @default.
• W2000966830 type Work @default.
• W2000966830 sameAs 2000966830 @default.
• W2000966830 citedByCount "5" @default.
• W2000966830 countsByYear W20009668302012 @default.
• W2000966830 crossrefType "journal-article" @default.
• W2000966830 hasAuthorship W2000966830A5032158458 @default.
• W2000966830 hasAuthorship W2000966830A5038479973 @default.
• W2000966830 hasAuthorship W2000966830A5089031929 @default.
• W2000966830 hasConcept C150757390 @default.
• W2000966830 hasConcept C181199279 @default.
• W2000966830 hasConcept C185592680 @default.
• W2000966830 hasConcept C2776980637 @default.
• W2000966830 hasConcept C2778078955 @default.
• W2000966830 hasConcept C2780857362 @default.
• W2000966830 hasConcept C55493867 @default.
• W2000966830 hasConcept C71240020 @default.
• W2000966830 hasConceptScore W2000966830C150757390 @default.
• W2000966830 hasConceptScore W2000966830C181199279 @default.
• W2000966830 hasConceptScore W2000966830C185592680 @default.
• W2000966830 hasConceptScore W2000966830C2776980637 @default.
• W2000966830 hasConceptScore W2000966830C2778078955 @default.
• W2000966830 hasConceptScore W2000966830C2780857362 @default.
• W2000966830 hasConceptScore W2000966830C55493867 @default.
• W2000966830 hasConceptScore W2000966830C71240020 @default.
• W2000966830 hasIssue "50" @default.
• W2000966830 hasLocation W20009668301 @default.
• W2000966830 hasLocation W20009668302 @default.
• W2000966830 hasOpenAccess W2000966830 @default.
• W2000966830 hasPrimaryLocation W20009668301 @default.
• W2000966830 hasRelatedWork W163649684 @default.
• W2000966830 hasRelatedWork W1994092422 @default.
• W2000966830 hasRelatedWork W2000966830 @default.
• W2000966830 hasRelatedWork W2009732597 @default.
• W2000966830 hasRelatedWork W2053729225 @default.
• W2000966830 hasRelatedWork W2120919525 @default.
• W2000966830 hasRelatedWork W2135491649 @default.
• W2000966830 hasRelatedWork W2510145288 @default.
• W2000966830 hasRelatedWork W2518790148 @default.
• W2000966830 hasRelatedWork W89645382 @default.
• W2000966830 hasVolume "38" @default.
• W2000966830 isParatext "false" @default.
• W2000966830 isRetracted "false" @default.
• W2000966830 magId "2000966830" @default.
• W2000966830 workType "article" @default.