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- W2000970044 abstract "Unspliced and partially spliced HIV RNAs are transported to the cytoplasm by the HIV encoded Rev protein. In the present study, a ribonucleoprotein complex which contains such incompletely spliced HIV RNA is identified. Soluble nuclear extracts were prepared from the lymphocyte cell line H9/IIIB that constitutively produces HIV-1 from a stably integrated provirus. Sucrose gradient centrifugation of the extracts and subsequent analysis of the gradient fractions by a ribonuclease protection assay revealed a population of incompletely spliced HIV-1 RNAs which accumulates in the 100S region of the gradient. Similar analysis of cellular mRNAs including β-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) revealed that these RNA molecules also exhibit characteristic sedimentation profiles in sucrose gradients. This study suggests that nuclear ribonucleoprotein particles containing incompletely spliced HIV-1 RNAs are amenable for biochemical characterisation." @default.
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- W2000970044 date "2000-02-01" @default.
- W2000970044 modified "2023-09-23" @default.
- W2000970044 title "Identification of a Nuclear Ribonucleoprotein Particle Which Contains Incompletely Spliced HIV-1 RNAs" @default.
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- W2000970044 doi "https://doi.org/10.1006/bbrc.2000.2177" @default.
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