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- W2000971934 abstract "The nuclear precursors of the immunoglobulin messenger RNAs of MPC-11 cells were characterized with respect to size, amount per cell and extent of polyadenylation. These cells produce three Ig mRNAs: a 1.8 kb component coding for a γ2b heavy chain (H mRNA), a 1.2 kb mRNA coding for a κ light chain (L mRNA) and a 0.8 kb mRNA coding for the constant region portion of the κ light chain (L1 mRNA). To identify the pre-mRNAs without ambiguity, we constructed recombinant DNA plasmids containing H and L cDNA sequences, and used the cloned cDNAs as hybridization probes for analysis of steady state nuclear RNA and in DNA excess hybridization experiments with pulse-labeled nuclear RNA. The nuclear molecules containing Ig sequences consist of an 11 kb component (H1), which we believe to be the primary transcript of the H gene, 5.3 kb (L1), and 3.3 kb (L2) components, which seem to be primary transcripts of the L and L1 genes, components corresponding to mature size H, L and L1 mRNAs, and several intermediatesized components which include the processing derivatives. The precursor role of these nuclear molecules was established by studies of their labeling kinetics and by appropriate pulse-chase experiments. All the pre-mRNA species including H1, L1 and L2 contain poly(A), thus suggesting that polyadenylation is an early event in the processing of these mRNAs. The MPC-11 cell contains about 30,000 and 40,000 cytoplasmic H and L mRNA molecules, respectively, which must be produced within one cell generation (∼24 hr). In comparison, the nucleus contains about 100–150 molecules of total pre-mRNA and only about 10–15 molecules of presumptive primary transcripts for each of these Ig species. These values indicate very rapid transcription rates (>20 transcripts per min) and exceptionally fast processing rates (∼0.5 min for the primary transcripts and ∼5 min for overall nuclear processing) for the Ig mRNAs. Thus rapid transcription and processing, together with high cytoplasmic stability, account for the high abundance of Ig mRNAs in the myeloma cell." @default.
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- W2000971934 date "1978-12-01" @default.
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- W2000971934 title "The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells" @default.
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- W2000971934 doi "https://doi.org/10.1016/0092-8674(78)90072-7" @default.
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