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• W2000976347 abstract "We describe a new procedure to Identify RNA or DNA binding sites in proteins, based on a combination of UV cross-linking and single-hit chemical peptlde cleavage. Site-directed mutagenesis is used to create a series of mutants with single Asn-Gly sequences in the protein to be analysed. Recombinant mutant proteins are incubated with their radiolabelled target sequence and UV Irradiated. Covalently linked RNA- or DNA-proteln complexes are digested with hydroxylamine and labelled peptides identified by SDS-PAGE and autoradiography. The analysis requires only small amounts of protein and is achieved within a relatively short time. Using this method we mapped the site at which human iron regulatory protein (IRP) is UV cross-linked to iron responsive element RNA to amino acid residues 116–151." @default.
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• W2000976347 date "1995-01-01" @default.
• W2000976347 modified "2023-10-18" @default.
• W2000976347 title "A novel method to identify nucleic acid binding sites in protiens by scanning mutagenesis: application to iron regulatory protein" @default.
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• W2000976347 doi "https://doi.org/10.1093/nar/23.14.2579" @default.
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