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• W2000977053 abstract "We report a simple and sensitive method for label-free detection of single-stranded DNA-binding protein (SSBP) based on an array of microfabricated cantilevers. The single-stranded DNA (ssDNA) was immobilized on the surface of the sensing cantilevers to detect SSBP, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. The differential deflection signals that reveal specific SSBP-ssDNA binding have been found to depend on the SSBP concentration. Using the cantilever array sensor we can detect SSBP in the concentration range from 0.01 to 7 μg mL(-1). Other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. Our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity." @default.
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• W2000977053 date "2013-05-01" @default.
• W2000977053 modified "2023-09-25" @default.
• W2000977053 title "Label-free detection of single-stranded DNA binding protein based on a cantilever array" @default.
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• W2000977053 doi "https://doi.org/10.1016/j.talanta.2013.02.001" @default.
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