FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000977138> ?p ?o ?g. }

• W2000977138 endingPage "1156" @default.
• W2000977138 startingPage "1147" @default.
• W2000977138 abstract "ObjectiveTo investigate the effects of repeated freezing and thawing on human sperm motility, vitality, and DNA integrity.DesignA prospective clinical study.SettingTertiary care fertility clinic.Patient(s)Twenty men presenting for infertility investigations.Intervention(s)Each sample was subjected to three cycles of freezing and thawing both with and without washing steps and the addition of fresh cryoprotectant between each cycle.Main Outcome Measure(s)Percentage sperm DNA fragmentation, motility, and vitality before and following repeated freezing and thawing.Result(s)The percentage sperm DNA fragmentation rose significantly following each freeze–thaw cycle; however, samples that were not washed and to which fresh cryoprotectant was not added after each cycle fared significantly better than their washed counterparts. Both motility and vitality decreased steadily following each cycle but cell survival was significantly greater in the unwashed samples.Conclusion(s)In terms of the level of sperm DNA fragmentation, up to three cycles of freezing and thawing can be performed with a level of risk comparable to that following a single cycle of freezing and thawing. This is provided that samples are refrozen in their original cryoprotectant and not washed or altered in any way in between, and provided that they are separated by density gradient centrifugation or swim-up before use in assisted reproduction technology. To investigate the effects of repeated freezing and thawing on human sperm motility, vitality, and DNA integrity. A prospective clinical study. Tertiary care fertility clinic. Twenty men presenting for infertility investigations. Each sample was subjected to three cycles of freezing and thawing both with and without washing steps and the addition of fresh cryoprotectant between each cycle. Percentage sperm DNA fragmentation, motility, and vitality before and following repeated freezing and thawing. The percentage sperm DNA fragmentation rose significantly following each freeze–thaw cycle; however, samples that were not washed and to which fresh cryoprotectant was not added after each cycle fared significantly better than their washed counterparts. Both motility and vitality decreased steadily following each cycle but cell survival was significantly greater in the unwashed samples. In terms of the level of sperm DNA fragmentation, up to three cycles of freezing and thawing can be performed with a level of risk comparable to that following a single cycle of freezing and thawing. This is provided that samples are refrozen in their original cryoprotectant and not washed or altered in any way in between, and provided that they are separated by density gradient centrifugation or swim-up before use in assisted reproduction technology." @default.
• W2000977138 created "2016-06-24" @default.
• W2000977138 creator A5006524940 @default.
• W2000977138 creator A5008471973 @default.
• W2000977138 creator A5018943158 @default.
• W2000977138 creator A5029740093 @default.
• W2000977138 creator A5078094747 @default.
• W2000977138 date "2010-03-01" @default.
• W2000977138 modified "2023-10-05" @default.
• W2000977138 title "The effect of repeated freezing and thawing on human sperm DNA fragmentation" @default.
• W2000977138 cites W1970692405 @default.
• W2000977138 cites W2005462057 @default.
• W2000977138 cites W2010231716 @default.
• W2000977138 cites W2020102787 @default.
• W2000977138 cites W2023957744 @default.
• W2000977138 cites W2024742837 @default.
• W2000977138 cites W2032704919 @default.
• W2000977138 cites W2063978777 @default.
• W2000977138 cites W2097286099 @default.
• W2000977138 cites W2109041986 @default.
• W2000977138 cites W2112435894 @default.
• W2000977138 cites W2115536254 @default.
• W2000977138 cites W2116932540 @default.
• W2000977138 cites W212117555 @default.
• W2000977138 cites W2132428901 @default.
• W2000977138 cites W2135622711 @default.
• W2000977138 cites W2140112227 @default.
• W2000977138 cites W2144136455 @default.
• W2000977138 cites W2149913116 @default.
• W2000977138 cites W2156101504 @default.
• W2000977138 cites W2159005332 @default.
• W2000977138 cites W2164175233 @default.
• W2000977138 cites W2166755477 @default.
• W2000977138 cites W2169140876 @default.
• W2000977138 cites W2240099669 @default.
• W2000977138 doi "https://doi.org/10.1016/j.fertnstert.2008.11.023" @default.
• W2000977138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19135665" @default.
• W2000977138 hasPublicationYear "2010" @default.
• W2000977138 type Work @default.
• W2000977138 sameAs 2000977138 @default.
• W2000977138 citedByCount "34" @default.
• W2000977138 countsByYear W20009771382012 @default.
• W2000977138 countsByYear W20009771382013 @default.
• W2000977138 countsByYear W20009771382014 @default.
• W2000977138 countsByYear W20009771382015 @default.
• W2000977138 countsByYear W20009771382016 @default.
• W2000977138 countsByYear W20009771382017 @default.
• W2000977138 countsByYear W20009771382018 @default.
• W2000977138 countsByYear W20009771382019 @default.
• W2000977138 countsByYear W20009771382020 @default.
• W2000977138 countsByYear W20009771382021 @default.
• W2000977138 crossrefType "journal-article" @default.
• W2000977138 hasAuthorship W2000977138A5006524940 @default.
• W2000977138 hasAuthorship W2000977138A5008471973 @default.
• W2000977138 hasAuthorship W2000977138A5018943158 @default.
• W2000977138 hasAuthorship W2000977138A5029740093 @default.
• W2000977138 hasAuthorship W2000977138A5078094747 @default.
• W2000977138 hasBestOaLocation W20009771381 @default.
• W2000977138 hasConcept C16685009 @default.
• W2000977138 hasConcept C179933525 @default.
• W2000977138 hasConcept C18903297 @default.
• W2000977138 hasConcept C190283241 @default.
• W2000977138 hasConcept C191015642 @default.
• W2000977138 hasConcept C196843134 @default.
• W2000977138 hasConcept C2777632594 @default.
• W2000977138 hasConcept C2777688143 @default.
• W2000977138 hasConcept C2778093475 @default.
• W2000977138 hasConcept C2779234561 @default.
• W2000977138 hasConcept C2779829227 @default.
• W2000977138 hasConcept C2781087480 @default.
• W2000977138 hasConcept C2908647359 @default.
• W2000977138 hasConcept C31573885 @default.
• W2000977138 hasConcept C43759708 @default.
• W2000977138 hasConcept C518429986 @default.
• W2000977138 hasConcept C54355233 @default.
• W2000977138 hasConcept C55493867 @default.
• W2000977138 hasConcept C58207958 @default.
• W2000977138 hasConcept C68528507 @default.
• W2000977138 hasConcept C71924100 @default.
• W2000977138 hasConcept C7623868 @default.
• W2000977138 hasConcept C86803240 @default.
• W2000977138 hasConcept C99454951 @default.
• W2000977138 hasConceptScore W2000977138C16685009 @default.
• W2000977138 hasConceptScore W2000977138C179933525 @default.
• W2000977138 hasConceptScore W2000977138C18903297 @default.
• W2000977138 hasConceptScore W2000977138C190283241 @default.
• W2000977138 hasConceptScore W2000977138C191015642 @default.
• W2000977138 hasConceptScore W2000977138C196843134 @default.
• W2000977138 hasConceptScore W2000977138C2777632594 @default.
• W2000977138 hasConceptScore W2000977138C2777688143 @default.
• W2000977138 hasConceptScore W2000977138C2778093475 @default.
• W2000977138 hasConceptScore W2000977138C2779234561 @default.
• W2000977138 hasConceptScore W2000977138C2779829227 @default.
• W2000977138 hasConceptScore W2000977138C2781087480 @default.
• W2000977138 hasConceptScore W2000977138C2908647359 @default.
• W2000977138 hasConceptScore W2000977138C31573885 @default.
• W2000977138 hasConceptScore W2000977138C43759708 @default.
• W2000977138 hasConceptScore W2000977138C518429986 @default.

• next