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- W2000978849 abstract "A procedure was adapted for the determination of cortisol and corticosterone in a single sample of peripheral blood plasma. The procedure consists of washing the plasma with isooctane, extracting it with dichloromethane, and washing the extract with sodium hydroxide. After removing acidic material the extract was evaporated and chromatographed on silica gel to separate cortisol from corticosterone. Corticosteroids were measured fluorometrically in an acid-alcohol mixture. Rigorous physiological and physicochemical checks were performed to demonstrate the specificity of the proposed procedure. The sensitivity and precision of the present method was superior to other routine fluorometric methods used to measure plasma corticosteroids. The level of cortisol and corticosterone in the peripheral plasma of six male rabbits averaged 1.9 and 6.0 μg/100 ml immediately after decapitation and 2.5 and 11.9 μg/100 ml following ether anesthesia. The procedure may be used to determine the concentration of cortisol and corticosterone in a sample of blood plasma providing the levels are above 5 mμg/ml." @default.
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- W2000978849 title "A quantitative procedure for the determination of cortisol and corticosterone in blood plasma" @default.
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- W2000978849 doi "https://doi.org/10.1016/s0039-128x(70)80110-6" @default.
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