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• W2000979009 startingPage "2207" @default.
• W2000979009 abstract "For the past 20 years, the increased development and routine application of molecular-based techniques has made it possible to carry out detailed evaluations of the biodiversity of aquatic microbial communities. It also offers great opportunities for finding out how this parameter responds to various environmental stresses. Most of these approaches involve an initial PCR amplification of a target, which is generally located within the ribosomal operon. The amplification is achieved by means of primers that are specific to the organisms of interest. The second step involves detecting sequence variations in the PCR fragments either by a cloning/sequencing analysis, which provides a complete characterization of the fragments, or by an electrophoretic analysis, which provides a visual separation of the mixture of fragments according to sequence polymorphism (denaturing or temperature gradient gel electrophoresis, single strand conformation polymorphism) or length polymorphism (terminal-restriction fragment length polymorphism, automated ribosomal intergenic spacer analysis). Other non-PCR-based methods are also commonly used, such as fluorescence in-situ hybridization and DNA re-association analysis. Depending on the technique used, the information gained can be quite different. Moreover, some of these analyses may be rather onerous in terms of time and money, and so not always suitable for screening large numbers of samples. The most widely used techniques are discussed in this paper to illustrate the principles, advantages and shortcomings of each of them. Finally, we will conclude by evaluating the techniques and discussing some emerging molecular techniques, such as real-time PCR and the microarray technique." @default.
• W2000979009 created "2016-06-24" @default.
• W2000979009 creator A5007738705 @default.
• W2000979009 creator A5040616126 @default.
• W2000979009 creator A5046923794 @default.
• W2000979009 date "2005-06-01" @default.
• W2000979009 modified "2023-09-27" @default.
• W2000979009 title "Molecular approaches to the assessment of biodiversity in aquatic microbial communities" @default.
• W2000979009 cites W1527271978 @default.
• W2000979009 cites W1555146534 @default.
• W2000979009 cites W1562239442 @default.
• W2000979009 cites W1577123626 @default.
• W2000979009 cites W1598574080 @default.
• W2000979009 cites W1684402650 @default.
• W2000979009 cites W1701869895 @default.
• W2000979009 cites W179716031 @default.
• W2000979009 cites W1870615197 @default.
• W2000979009 cites W1883956239 @default.
• W2000979009 cites W1907872891 @default.
• W2000979009 cites W1958758746 @default.
• W2000979009 cites W1967901188 @default.
• W2000979009 cites W1968488194 @default.
• W2000979009 cites W1971635124 @default.
• W2000979009 cites W1973531600 @default.
• W2000979009 cites W1977993587 @default.
• W2000979009 cites W1986323506 @default.
• W2000979009 cites W1986706966 @default.
• W2000979009 cites W1988660274 @default.
• W2000979009 cites W1993163345 @default.
• W2000979009 cites W1993602546 @default.
• W2000979009 cites W1994930035 @default.
• W2000979009 cites W1995826336 @default.
• W2000979009 cites W1996027687 @default.
• W2000979009 cites W1997351298 @default.
• W2000979009 cites W2000786883 @default.
• W2000979009 cites W2003486206 @default.
• W2000979009 cites W2006887501 @default.
• W2000979009 cites W2007250847 @default.
• W2000979009 cites W2010570381 @default.
• W2000979009 cites W2010680821 @default.
• W2000979009 cites W2011490670 @default.
• W2000979009 cites W2013522623 @default.
• W2000979009 cites W2018150478 @default.
• W2000979009 cites W2019374755 @default.
• W2000979009 cites W2020140162 @default.
• W2000979009 cites W2025848452 @default.
• W2000979009 cites W2027600997 @default.
• W2000979009 cites W2028829346 @default.
• W2000979009 cites W2030784825 @default.
• W2000979009 cites W2031274779 @default.
• W2000979009 cites W2034428861 @default.
• W2000979009 cites W2035775871 @default.
• W2000979009 cites W2036389069 @default.
• W2000979009 cites W2036713421 @default.
• W2000979009 cites W2040653294 @default.
• W2000979009 cites W2047888982 @default.
• W2000979009 cites W2049449067 @default.
• W2000979009 cites W2051497662 @default.
• W2000979009 cites W2055305007 @default.
• W2000979009 cites W2061472576 @default.
• W2000979009 cites W2064974028 @default.
• W2000979009 cites W2067297267 @default.
• W2000979009 cites W2068687524 @default.
• W2000979009 cites W2069113548 @default.
• W2000979009 cites W2072212611 @default.
• W2000979009 cites W2072556768 @default.
• W2000979009 cites W2074745628 @default.
• W2000979009 cites W2079268915 @default.
• W2000979009 cites W2079446230 @default.
• W2000979009 cites W2083621226 @default.
• W2000979009 cites W2086219374 @default.
• W2000979009 cites W2090355482 @default.
• W2000979009 cites W2097009480 @default.
• W2000979009 cites W2097033182 @default.
• W2000979009 cites W2101577464 @default.
• W2000979009 cites W2101828188 @default.
• W2000979009 cites W2102576392 @default.
• W2000979009 cites W2104200271 @default.
• W2000979009 cites W2105317669 @default.
• W2000979009 cites W2107974372 @default.
• W2000979009 cites W2111002704 @default.
• W2000979009 cites W2111858111 @default.
• W2000979009 cites W2112152978 @default.
• W2000979009 cites W2116644543 @default.
• W2000979009 cites W2119166302 @default.
• W2000979009 cites W2120108422 @default.
• W2000979009 cites W2120917878 @default.
• W2000979009 cites W2124179074 @default.
• W2000979009 cites W2125862549 @default.
• W2000979009 cites W2126263950 @default.
• W2000979009 cites W2126862456 @default.
• W2000979009 cites W2128001070 @default.
• W2000979009 cites W2130595252 @default.
• W2000979009 cites W2130954747 @default.
• W2000979009 cites W2131954892 @default.
• W2000979009 cites W2132088881 @default.
• W2000979009 cites W2132853588 @default.
• W2000979009 cites W2133043748 @default.

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