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- W2000983544 abstract "We report the identification of a family of four active genes ( Ehodp1 , Ehodp2 , Ehodp3, and Ehodp4 ) encoding putative DNA polymerases in Entamoeba histolytica , the protozoan parasite responsible of human amoebiasis. The four Ehodp genes show similarity to DNA polymerases encoded in fungi and plant mitochondrial plasmids. EhODP polypeptides conserve the<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML><mml:mrow><mml:msup><mml:mn>3</mml:mn><mml:mo>′</mml:mo></mml:msup><mml:mtext>-</mml:mtext><mml:msup><mml:mn>5</mml:mn><mml:mo>′</mml:mo></mml:msup></mml:mrow></mml:math>exonuclease II and<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML><mml:mrow><mml:msup><mml:mn>5</mml:mn><mml:mo>′</mml:mo></mml:msup><mml:mtext>-</mml:mtext><mml:msup><mml:mn>3</mml:mn><mml:mo>′</mml:mo></mml:msup></mml:mrow></mml:math>polymerization domains, and they have the I, II, and III conserved boxes that characterize them as DNA polymerases of family B. Furthermore, we found in EhODP polymerases two novel A and B boxes, present also in DNA polymerases encoded in fungi mitochondrial plasmids. By in situ PCR, Ehodp1 gene was located in nuclei and in DNA-containing cytoplasmic structures. Additionally, using polyclonal antibodies against a recombinant rEhODP1-168 polypeptide, and confocal microscopy, EhODP1 was located in cytoplasmic DNA-containing structures." @default.
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- W2000983544 date "2010-01-01" @default.
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- W2000983544 title "Identification of FourEntamoeba histolyticaOrganellar DNA Polymerases of the Family B and Cellular Localization of theEhodp1Gene and EhODP1 Protein" @default.
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- W2000983544 doi "https://doi.org/10.1155/2010/734898" @default.
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