Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000985029> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2000985029 endingPage "2940" @default.
- W2000985029 startingPage "2936" @default.
- W2000985029 abstract "Monoclonal antibodies were prepared against nisin A (nisA), an antimicrobial peptide that is used as a food preservative and is produced by strains of Lactococcus lactis subsp. lactis. NisA antibodies were secreted by hybridomas derived from mice immunized with a nisA−keyhole limpet hemocyanin conjugate. Competitive direct enzyme-linked immunoabsorbent assays (CD-ELISA) were devised whereby free nisA and nisA−horseradish peroxidase competed for antibody binding. The detection limit for nisA in this assay was 10 ng/mL. Antibodies also cross-reacted with nisin Z (nisZ). Mean concentrations of nisA and nisZ required to inhibit 50% antibody binding for the selected clones were 1180 and 3750 ng/mL, respectively. When the nisA CD-ELISA performance was assessed in spiked (250, 1250, and 5000 ng/g) spread cheese, the average recovery was 116.5%, with mean intra- and interassay coefficients of variation of 13.3 and 14.1%, respectively. Keywords: Nisin A; monoclonal antibodies; ELISA; lantibiotics; bacteriocins" @default.
- W2000985029 created "2016-06-24" @default.
- W2000985029 creator A5003338339 @default.
- W2000985029 creator A5016527076 @default.
- W2000985029 creator A5021906177 @default.
- W2000985029 creator A5058788884 @default.
- W2000985029 creator A5067032163 @default.
- W2000985029 date "1996-01-01" @default.
- W2000985029 modified "2023-10-02" @default.
- W2000985029 title "Development of Monoclonal Antibodies to the Lantibiotic Nisin A" @default.
- W2000985029 cites W1563155242 @default.
- W2000985029 cites W1980900837 @default.
- W2000985029 cites W2070283605 @default.
- W2000985029 cites W2080477691 @default.
- W2000985029 cites W2117533139 @default.
- W2000985029 cites W2155045365 @default.
- W2000985029 cites W2170055655 @default.
- W2000985029 doi "https://doi.org/10.1021/jf9602232" @default.
- W2000985029 hasPublicationYear "1996" @default.
- W2000985029 type Work @default.
- W2000985029 sameAs 2000985029 @default.
- W2000985029 citedByCount "20" @default.
- W2000985029 countsByYear W20009850292012 @default.
- W2000985029 crossrefType "journal-article" @default.
- W2000985029 hasAuthorship W2000985029A5003338339 @default.
- W2000985029 hasAuthorship W2000985029A5016527076 @default.
- W2000985029 hasAuthorship W2000985029A5021906177 @default.
- W2000985029 hasAuthorship W2000985029A5058788884 @default.
- W2000985029 hasAuthorship W2000985029A5067032163 @default.
- W2000985029 hasConcept C132141352 @default.
- W2000985029 hasConcept C153911025 @default.
- W2000985029 hasConcept C159654299 @default.
- W2000985029 hasConcept C185592680 @default.
- W2000985029 hasConcept C203014093 @default.
- W2000985029 hasConcept C2775920511 @default.
- W2000985029 hasConcept C2776727803 @default.
- W2000985029 hasConcept C2778457984 @default.
- W2000985029 hasConcept C2780011460 @default.
- W2000985029 hasConcept C4937899 @default.
- W2000985029 hasConcept C523546767 @default.
- W2000985029 hasConcept C542903549 @default.
- W2000985029 hasConcept C54355233 @default.
- W2000985029 hasConcept C86803240 @default.
- W2000985029 hasConcept C89423630 @default.
- W2000985029 hasConceptScore W2000985029C132141352 @default.
- W2000985029 hasConceptScore W2000985029C153911025 @default.
- W2000985029 hasConceptScore W2000985029C159654299 @default.
- W2000985029 hasConceptScore W2000985029C185592680 @default.
- W2000985029 hasConceptScore W2000985029C203014093 @default.
- W2000985029 hasConceptScore W2000985029C2775920511 @default.
- W2000985029 hasConceptScore W2000985029C2776727803 @default.
- W2000985029 hasConceptScore W2000985029C2778457984 @default.
- W2000985029 hasConceptScore W2000985029C2780011460 @default.
- W2000985029 hasConceptScore W2000985029C4937899 @default.
- W2000985029 hasConceptScore W2000985029C523546767 @default.
- W2000985029 hasConceptScore W2000985029C542903549 @default.
- W2000985029 hasConceptScore W2000985029C54355233 @default.
- W2000985029 hasConceptScore W2000985029C86803240 @default.
- W2000985029 hasConceptScore W2000985029C89423630 @default.
- W2000985029 hasIssue "9" @default.
- W2000985029 hasLocation W20009850291 @default.
- W2000985029 hasOpenAccess W2000985029 @default.
- W2000985029 hasPrimaryLocation W20009850291 @default.
- W2000985029 hasRelatedWork W2000985029 @default.
- W2000985029 hasRelatedWork W2016957978 @default.
- W2000985029 hasRelatedWork W2040776903 @default.
- W2000985029 hasRelatedWork W2051531983 @default.
- W2000985029 hasRelatedWork W2091276993 @default.
- W2000985029 hasRelatedWork W2120631617 @default.
- W2000985029 hasRelatedWork W2171918546 @default.
- W2000985029 hasRelatedWork W4247789171 @default.
- W2000985029 hasRelatedWork W580498224 @default.
- W2000985029 hasRelatedWork W3111876846 @default.
- W2000985029 hasVolume "44" @default.
- W2000985029 isParatext "false" @default.
- W2000985029 isRetracted "false" @default.
- W2000985029 magId "2000985029" @default.
- W2000985029 workType "article" @default.