FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000989654> ?p ?o ?g. }

• W2000989654 endingPage "e75119" @default.
• W2000989654 startingPage "e75119" @default.
• W2000989654 abstract "Among the neuronal binding partners of calmodulin (CaM) are Munc13 proteins as essential presynaptic regulators that play a key role in synaptic vesicle priming and are crucial for presynaptic short-term plasticity. Recent NMR structural investigations of a CaM/Munc13-1 peptide complex have revealed an extended structure, which contrasts the compact structures of most classical CaM/target complexes. This unusual binding mode is thought to be related to the presence of an additional hydrophobic anchor residue at position 26 of the CaM binding motif of Munc13-1, resulting in a novel 1-5-8-26 motif. Here, we addressed the question whether the 1-5-8-26 CaM binding motif is a Munc13-related feature or whether it can be induced in other CaM targets by altering the motif's core residues. For this purpose, we chose skeletal muscle myosin light chain kinase (skMLCK) with a classical 1-5-8-14 CaM binding motif and constructed three skMLCK peptide variants mimicking Munc13-1, in which the hydrophobic anchor amino acid at position 14 was moved to position 26. Chemical cross-linking between CaM and skMLCK peptide variants combined with high-resolution mass spectrometry yielded insights into the peptides' binding modes. This structural comparison together with complementary binding data from surface plasmon resonance experiments revealed that skMLCK variants with an artificial 1-5-8-26 motif cannot mimic CaM binding of Munc13-1. Apparently, additional features apart from the spacing of the hydrophobic anchor residues are required to define the functional 1-5-8-26 motif of Munc13-1. We conclude that Munc13 proteins display a unique CaM binding behavior to fulfill their role as efficient presynaptic calcium sensors over broad range of Ca2+ concentrations." @default.
• W2000989654 created "2016-06-24" @default.
• W2000989654 creator A5048986823 @default.
• W2000989654 creator A5064614134 @default.
• W2000989654 creator A5076101506 @default.
• W2000989654 creator A5076158259 @default.
• W2000989654 creator A5076952708 @default.
• W2000989654 creator A5084158849 @default.
• W2000989654 date "2013-10-10" @default.
• W2000989654 modified "2023-09-27" @default.
• W2000989654 title "Munc13-Like skMLCK Variants Cannot Mimic the Unique Calmodulin Binding Mode of Munc13 as Evidenced by Chemical Cross-Linking and Mass Spectrometry" @default.
• W2000989654 cites W1618714882 @default.
• W2000989654 cites W1963910961 @default.
• W2000989654 cites W1975271855 @default.
• W2000989654 cites W1980485487 @default.
• W2000989654 cites W1983717205 @default.
• W2000989654 cites W1985788979 @default.
• W2000989654 cites W1992332602 @default.
• W2000989654 cites W1994684691 @default.
• W2000989654 cites W1997913941 @default.
• W2000989654 cites W1998747769 @default.
• W2000989654 cites W2012881492 @default.
• W2000989654 cites W2018272940 @default.
• W2000989654 cites W2024142216 @default.
• W2000989654 cites W2030490793 @default.
• W2000989654 cites W2031513106 @default.
• W2000989654 cites W2032069841 @default.
• W2000989654 cites W2034942562 @default.
• W2000989654 cites W2044228723 @default.
• W2000989654 cites W2050024578 @default.
• W2000989654 cites W2050696429 @default.
• W2000989654 cites W2058712930 @default.
• W2000989654 cites W2059054942 @default.
• W2000989654 cites W2061342956 @default.
• W2000989654 cites W2063150400 @default.
• W2000989654 cites W2079916233 @default.
• W2000989654 cites W2087087669 @default.
• W2000989654 cites W2090657212 @default.
• W2000989654 cites W2100837269 @default.
• W2000989654 cites W2132138394 @default.
• W2000989654 cites W2133380824 @default.
• W2000989654 cites W2155341464 @default.
• W2000989654 doi "https://doi.org/10.1371/journal.pone.0075119" @default.
• W2000989654 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3794949" @default.
• W2000989654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24130683" @default.
• W2000989654 hasPublicationYear "2013" @default.
• W2000989654 type Work @default.
• W2000989654 sameAs 2000989654 @default.
• W2000989654 citedByCount "4" @default.
• W2000989654 countsByYear W20009896542014 @default.
• W2000989654 countsByYear W20009896542017 @default.
• W2000989654 countsByYear W20009896542020 @default.
• W2000989654 crossrefType "journal-article" @default.
• W2000989654 hasAuthorship W2000989654A5048986823 @default.
• W2000989654 hasAuthorship W2000989654A5064614134 @default.
• W2000989654 hasAuthorship W2000989654A5076101506 @default.
• W2000989654 hasAuthorship W2000989654A5076158259 @default.
• W2000989654 hasAuthorship W2000989654A5076952708 @default.
• W2000989654 hasAuthorship W2000989654A5084158849 @default.
• W2000989654 hasBestOaLocation W20009896541 @default.
• W2000989654 hasConcept C104317684 @default.
• W2000989654 hasConcept C106847996 @default.
• W2000989654 hasConcept C107824862 @default.
• W2000989654 hasConcept C12554922 @default.
• W2000989654 hasConcept C132677234 @default.
• W2000989654 hasConcept C155672457 @default.
• W2000989654 hasConcept C167625842 @default.
• W2000989654 hasConcept C171250308 @default.
• W2000989654 hasConcept C181199279 @default.
• W2000989654 hasConcept C185592680 @default.
• W2000989654 hasConcept C192562407 @default.
• W2000989654 hasConcept C2779281246 @default.
• W2000989654 hasConcept C29688787 @default.
• W2000989654 hasConcept C51639874 @default.
• W2000989654 hasConcept C55493867 @default.
• W2000989654 hasConcept C71240020 @default.
• W2000989654 hasConcept C86803240 @default.
• W2000989654 hasConceptScore W2000989654C104317684 @default.
• W2000989654 hasConceptScore W2000989654C106847996 @default.
• W2000989654 hasConceptScore W2000989654C107824862 @default.
• W2000989654 hasConceptScore W2000989654C12554922 @default.
• W2000989654 hasConceptScore W2000989654C132677234 @default.
• W2000989654 hasConceptScore W2000989654C155672457 @default.
• W2000989654 hasConceptScore W2000989654C167625842 @default.
• W2000989654 hasConceptScore W2000989654C171250308 @default.
• W2000989654 hasConceptScore W2000989654C181199279 @default.
• W2000989654 hasConceptScore W2000989654C185592680 @default.
• W2000989654 hasConceptScore W2000989654C192562407 @default.
• W2000989654 hasConceptScore W2000989654C2779281246 @default.
• W2000989654 hasConceptScore W2000989654C29688787 @default.
• W2000989654 hasConceptScore W2000989654C51639874 @default.
• W2000989654 hasConceptScore W2000989654C55493867 @default.
• W2000989654 hasConceptScore W2000989654C71240020 @default.
• W2000989654 hasConceptScore W2000989654C86803240 @default.
• W2000989654 hasIssue "10" @default.
• W2000989654 hasLocation W20009896541 @default.
• W2000989654 hasLocation W20009896542 @default.
• W2000989654 hasLocation W20009896543 @default.

• next