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- W2000990710 abstract "Changes in the antigenic constitutions of the kidney cells of the chicken embryos during primary culture in monolayer condition were studied by means of (1) in vitro agar diffusion test and quantitative precipitin test and (2) antibody staining method. The antigenic components in the post-microsomal supernatant are maintained at least up to the final stage of primary culture, while those contained in the microsomal fraction tend to decrease during this period. The changes occurring in the microsomal “kidney-specific” antigens (K-antigens) were studied in more detail. The average content per individual “K-antigen-containing” cell was decreased by about half between 0 and 7 days of primary culture. K-antigens which were localized only in the apical cytoplasm in the precultured tissue cells were found to be diffusely distributed throughout the cytoplasm in older cultures. Furthermore, the present paper showed the presence of the “culture antigens” which were detectable exclusively in older cultures. Such changes occurring in primary culture are considered not to be due only to a selection of particular type of cells but mainly due to phenotypic changes of the cellular immunological characteristics." @default.
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- W2000990710 date "1965-09-01" @default.
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- W2000990710 title "Changes in antigenic constitutions of embryonic chicken kidney cells during spreading culture" @default.
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- W2000990710 doi "https://doi.org/10.1016/0014-4827(65)90061-3" @default.
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