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• W2000990883 abstract "Abstract We aimed to develop a reliable method based on Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) to discriminate Escherichia coli clones from B2(n = 9) and D(n = 13) phylogenetic groups. Eighty-eight E. coli isolates belonging to phylogenetic groups B2(n = 39) and D(n = 49), including particularly widespread high risk clones or clonal complexes (HiRCC) ST131, ST69, ST393 and ST405 were studied. Spectra were analysed by unsupervised (hierarchical cluster analysis-HCA) and supervised methods (soft independent modelling of class analogy-SIMCA and partial least square discriminant analysis-PLSDA). B2-ST131 isolates were discriminated from B2 non-ST131 and D phylogroup isolates (ST69, ST393, ST405) by HCA, SIMCA and PLSDA. D-ST69, D-ST393 and D-ST405 isolates were also distinguished from each other and from other STs from phylogroup D by the three methods. We demonstrate that FTIR-ATR coupled with chemometrics is a reliable and alternative method to accurately discriminate particular E. coli clones. Its validation towards an application at a routine basis could revolutionize high-throughput bacterial typing." @default.
• W2000990883 created "2016-06-24" @default.
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• W2000990883 date "2013-11-20" @default.
• W2000990883 modified "2023-09-26" @default.
• W2000990883 title "Diverse high-risk B2 and D Escherichia coli clones depicted by Fourier Transform Infrared Spectroscopy" @default.
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• W2000990883 doi "https://doi.org/10.1038/srep03278" @default.
• W2000990883 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3834554" @default.
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