FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000991699> ?p ?o ?g. }

• W2000991699 endingPage "209" @default.
• W2000991699 startingPage "197" @default.
• W2000991699 abstract "Interspecies hybrids were constructed by fusing normal human male peripheral blood mononuclear cells with BW5147, a HGPRTt thymoma derived from an AKR mouse. Hybrid cells were selected in HAT media in culture dishes containing 1 × 107 human red blood cells. Twelve weeks after fusion, hybridomas were diluted to 10–15 cells/well and characterized for their expression of the human immune cell surface antigens HLA-DR, T3, T4, and T8 using fluorescent microscopy and cytographic analysis. More than 70% of the hybrid colonies expressed human T-cell surface antigens. Moreover the specific human repetitive DNA (ALU) bound to DNA sequences isolated from the hybridomas after Southern transfers. However, the same hybrids did not have a statistically significant increase in their chromosome number when compared to the mouse parent cell line. Several of the hybridomas produced a soluble factor capable of stimulating the growth of the IL-2 restricted murine cell line CTLL-2 and supported DNA synthesis in human peripheral T-cell populations. Panning experiments demonstrated that the IL-2 producing hybridomas could be enriched by selecting for the human T-cell surface antigen T3. The results presented here indicate that mouse × human hybridomas which express a broad range of human lymphocyte markers can be constructed and maintained in continuous culture for extended periods of time. It also appears that the T3-Ti receptor complex mediates the proliferation of T cells through the T3 molecules linkage to the secretion and/or production of IL-2. The usefulness of interspecific T-cell hybrids as an immunogenetic research tool as well as the significance of the mapping data are discussed." @default.
• W2000991699 created "2016-06-24" @default.
• W2000991699 creator A5023265367 @default.
• W2000991699 creator A5062480352 @default.
• W2000991699 creator A5076242074 @default.
• W2000991699 creator A5090235621 @default.
• W2000991699 date "1986-06-01" @default.
• W2000991699 modified "2023-09-25" @default.
• W2000991699 title "Expression of human T antigens in interspecies hybridomas" @default.
• W2000991699 cites W1506157243 @default.
• W2000991699 cites W1601344030 @default.
• W2000991699 cites W1981471956 @default.
• W2000991699 cites W1990108802 @default.
• W2000991699 cites W1996249519 @default.
• W2000991699 cites W2008384204 @default.
• W2000991699 cites W2012006189 @default.
• W2000991699 cites W2017107762 @default.
• W2000991699 cites W2021564912 @default.
• W2000991699 cites W2023646213 @default.
• W2000991699 cites W2024943390 @default.
• W2000991699 cites W2044220392 @default.
• W2000991699 cites W2046208949 @default.
• W2000991699 cites W2054320393 @default.
• W2000991699 cites W2056723350 @default.
• W2000991699 cites W2061227314 @default.
• W2000991699 cites W2061241669 @default.
• W2000991699 cites W2063450941 @default.
• W2000991699 cites W2064418850 @default.
• W2000991699 cites W2065961289 @default.
• W2000991699 cites W2066251506 @default.
• W2000991699 cites W2087116617 @default.
• W2000991699 cites W2091028925 @default.
• W2000991699 cites W2104453235 @default.
• W2000991699 cites W2133508418 @default.
• W2000991699 cites W2136346667 @default.
• W2000991699 cites W2136941426 @default.
• W2000991699 cites W2137581909 @default.
• W2000991699 cites W2167936032 @default.
• W2000991699 doi "https://doi.org/10.1016/0008-8749(86)90019-5" @default.
• W2000991699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2943408" @default.
• W2000991699 hasPublicationYear "1986" @default.
• W2000991699 type Work @default.
• W2000991699 sameAs 2000991699 @default.
• W2000991699 citedByCount "8" @default.
• W2000991699 countsByYear W20009916992012 @default.
• W2000991699 crossrefType "journal-article" @default.
• W2000991699 hasAuthorship W2000991699A5023265367 @default.
• W2000991699 hasAuthorship W2000991699A5062480352 @default.
• W2000991699 hasAuthorship W2000991699A5076242074 @default.
• W2000991699 hasAuthorship W2000991699A5090235621 @default.
• W2000991699 hasConcept C147483822 @default.
• W2000991699 hasConcept C153911025 @default.
• W2000991699 hasConcept C203014093 @default.
• W2000991699 hasConcept C2776090121 @default.
• W2000991699 hasConcept C2992881475 @default.
• W2000991699 hasConcept C54355233 @default.
• W2000991699 hasConcept C81885089 @default.
• W2000991699 hasConcept C86803240 @default.
• W2000991699 hasConcept C8891405 @default.
• W2000991699 hasConceptScore W2000991699C147483822 @default.
• W2000991699 hasConceptScore W2000991699C153911025 @default.
• W2000991699 hasConceptScore W2000991699C203014093 @default.
• W2000991699 hasConceptScore W2000991699C2776090121 @default.
• W2000991699 hasConceptScore W2000991699C2992881475 @default.
• W2000991699 hasConceptScore W2000991699C54355233 @default.
• W2000991699 hasConceptScore W2000991699C81885089 @default.
• W2000991699 hasConceptScore W2000991699C86803240 @default.
• W2000991699 hasConceptScore W2000991699C8891405 @default.
• W2000991699 hasIssue "1" @default.
• W2000991699 hasLocation W20009916991 @default.
• W2000991699 hasLocation W20009916992 @default.
• W2000991699 hasOpenAccess W2000991699 @default.
• W2000991699 hasPrimaryLocation W20009916991 @default.
• W2000991699 hasRelatedWork W2001055320 @default.
• W2000991699 hasRelatedWork W2031356189 @default.
• W2000991699 hasRelatedWork W2085713490 @default.
• W2000991699 hasRelatedWork W2141965224 @default.
• W2000991699 hasRelatedWork W2149871822 @default.
• W2000991699 hasRelatedWork W2220102944 @default.
• W2000991699 hasRelatedWork W2409441623 @default.
• W2000991699 hasRelatedWork W402048087 @default.
• W2000991699 hasRelatedWork W4210976760 @default.
• W2000991699 hasRelatedWork W74037808 @default.
• W2000991699 hasVolume "100" @default.
• W2000991699 isParatext "false" @default.
• W2000991699 isRetracted "false" @default.
• W2000991699 magId "2000991699" @default.
• W2000991699 workType "article" @default.