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• W2000995367 abstract "A method is described for the determination of low plasma levels of adenosine-5′-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 μmol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 ± 0.025 (10) μmol/liter and rabbit plasma concentration were 0.07 ± 0.05 (5) μmol/liter. Several other possible applications of the method are discussed." @default.
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• W2000995367 date "1978-01-01" @default.
• W2000995367 modified "2023-09-25" @default.
• W2000995367 title "Plasma ADP levels: Direct determination with luciferase luminescence using a biometer" @default.
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• W2000995367 doi "https://doi.org/10.1016/s0009-9120(78)80027-7" @default.
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