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• W2000998466 startingPage "5578" @default.
• W2000998466 abstract "Efforts to further the potential of the large perennial grass Miscanthus × giganteus as a biofuel feedstock would be aided by the ability to image the chemical species present during the fuel production process. Toward this end, two mass spectrometry imaging (MSI) approaches have been investigated here—laser desorption/ionization mass spectrometry (LDI-MS) and secondary ion mass spectrometry (SIMS). As a first step, cross sections of Miscanthus were subjected to a variety of sample preparation methods to optimize conditions for MSI. For LDI-MS, a thin metal coating (2 nm thick Au) provided high quality signals of saccharide-related ions. The traditional matrix-assisted laser desorption/ionization matrix, 2,5-dihydroxybenzoic acid, also showed high efficiency for the desorption of saccharide-related ions. In contrast, with α-cyano-4-hydroxycinnamic acid matrix, these ions were nearly absent in the mass spectra. Direct laser ablation of untreated Miscanthus sections was also performed. High resolution images, where the fine structure of the vascular bundle could be clearly visualized, were obtained using SIMS. Although coating the sections with a nanometer thick Au layer can greatly enhance the quality of SIMS images, the coating had limited effect on secondary ion signal enhancement. Using the optimized mass spectrometry approaches described here, information on the spatial distribution of several saccharides was obtained." @default.
• W2000998466 created "2016-06-24" @default.
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• W2000998466 creator A5010444377 @default.
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• W2000998466 date "2010-07-01" @default.
• W2000998466 modified "2023-09-22" @default.
• W2000998466 title "Comparison of sample pre-treatments for laser desorption ionization and secondary ion mass spectrometry imaging of Miscanthus×giganteus" @default.
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• W2000998466 doi "https://doi.org/10.1016/j.biortech.2010.01.136" @default.
• W2000998466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20171881" @default.
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