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- W2000999634 abstract "A set of protein fragments was produced by site-directed mutagenesis followed by chemical cleavage of phosphoglycerate kinase according to a previously described method [Pecorari et al. (1993) Protein Eng. 6, 313−325]. The cleavage positions were chosen in order to correspond to limits between structural subdomains. These isolated fragments were studied by circular dichroism, folding transitions, and cross-linking analyses. It appears that fragments corresponding to globular subdomains in the protein can recover the expected helix content. However, the cooperativity classically observed in the folding transitions of natural proteins is only observed for fragments larger than a domain. Previous studies have shown that the isolated C-terminal domain is an autonomous folding unit which displays a single cooperative transition [Missiakas et al. (1990) Biochemistry 29, 8683−8689]. The results presented here show that the presence in a fragment of a sequence overpassing that of the C-terminal domain modifies its folding process. Reassociation experiments suggest that the efficiency of the complementation process is not related to the folding autonomy of the isolated fragments." @default.
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- W2000999634 date "1996-01-01" @default.
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- W2000999634 title "Folding and Functional Complementation of Engineered Fragments from Yeast Phosphoglycerate Kinase" @default.
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- W2000999634 doi "https://doi.org/10.1021/bi951973s" @default.
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