FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001032368> ?p ?o ?g. }

• W2001032368 endingPage "989" @default.
• W2001032368 startingPage "981" @default.
• W2001032368 abstract "Putrescine uptake in type II pneumocytes is a carrier-mediated active process. Our hypothesis was that oligoamines might be taken up into the cell at least in part by gamma-glutamyltransferase (gamma-GT). This was investigated in rat type II pneumocytes 24 hr after their isolation. Preexposure to 125 microM L-buthionine-[SR]-sulfoximine (BSO) or 100 microM diethylmaleate (DEM), both of which affect intracellular glutathione (GSH) only, were found to decrease GSH by 85% (p < 0.05) and 62%, respectively (p < 0.05), without change in [3H]-putrescine uptake. Preexposure to 20 microM N-ethylmaleimide (NEM), which affects intra- and extracellular GSH, decreased intracellular GSH by 79% (p = 0.015) and putrescine uptake by 39% (p = 0.03). Selective extracellular GSH depletion by 10 microM copper-o-phenanthroline complex (CuP) led to a decrease in putrescine uptake of 41% (p = 0.001), while intracellular GSH remained unchanged. Specific inhibition of gamma-GT by 5-20 mM serine-borate or 5 mM acivicin gave similar degrees of putrescine uptake inhibition (39.5% and 40.5%). The kinetic properties of the putrescine uptake system in the presence of acivicin and serine-borate indicated that the Vmax decreased by 25%, while Km remained unchanged. In experiments with pure gamma-GT, the oligoamines putrescine, spermidine and spermine, and cystamine proved to be acceptor substrates for gamma-GT, all having similar efficiencies (Vmax/Km); methylglyoxal-bis-(guanyl-hydrazone) and paraquat were not accepted. As extracellular GSH is required for gamma-GT, and because its extracellular depletion inhibits putrescine uptake as much as specific inhibition of gamma-GT, we suggest that 30-40% of the putrescine uptake in type II pneumocytes occurs by gamma-GT and that, therefore, at least two systems are involved in the uptake of putrescine." @default.
• W2001032368 created "2016-06-24" @default.
• W2001032368 creator A5053106594 @default.
• W2001032368 creator A5061521668 @default.
• W2001032368 creator A5071543439 @default.
• W2001032368 creator A5086634345 @default.
• W2001032368 date "1995-09-01" @default.
• W2001032368 modified "2023-10-17" @default.
• W2001032368 title "Role of γ-glutamyltransferase in putrescine uptake by rat type II pneumocytes" @default.
• W2001032368 cites W1199623702 @default.
• W2001032368 cites W1223981148 @default.
• W2001032368 cites W1500346713 @default.
• W2001032368 cites W1502544270 @default.
• W2001032368 cites W1633567371 @default.
• W2001032368 cites W1648994921 @default.
• W2001032368 cites W1784456916 @default.
• W2001032368 cites W1979391388 @default.
• W2001032368 cites W1994778106 @default.
• W2001032368 cites W1995604250 @default.
• W2001032368 cites W1998767256 @default.
• W2001032368 cites W2001321803 @default.
• W2001032368 cites W2012026340 @default.
• W2001032368 cites W2021256316 @default.
• W2001032368 cites W2042372525 @default.
• W2001032368 cites W2043985253 @default.
• W2001032368 cites W2045674037 @default.
• W2001032368 cites W2050116877 @default.
• W2001032368 cites W2054733181 @default.
• W2001032368 cites W2061440119 @default.
• W2001032368 cites W2069874620 @default.
• W2001032368 cites W2082629145 @default.
• W2001032368 cites W2340271041 @default.
• W2001032368 cites W2415906399 @default.
• W2001032368 cites W4245957869 @default.
• W2001032368 cites W4293247451 @default.
• W2001032368 cites W758260955 @default.
• W2001032368 cites W2053476668 @default.
• W2001032368 doi "https://doi.org/10.1016/0006-2952(95)00223-m" @default.
• W2001032368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7575683" @default.
• W2001032368 hasPublicationYear "1995" @default.
• W2001032368 type Work @default.
• W2001032368 sameAs 2001032368 @default.
• W2001032368 citedByCount "6" @default.
• W2001032368 crossrefType "journal-article" @default.
• W2001032368 hasAuthorship W2001032368A5053106594 @default.
• W2001032368 hasAuthorship W2001032368A5061521668 @default.
• W2001032368 hasAuthorship W2001032368A5071543439 @default.
• W2001032368 hasAuthorship W2001032368A5086634345 @default.
• W2001032368 hasConcept C153911025 @default.
• W2001032368 hasConcept C181199279 @default.
• W2001032368 hasConcept C185592680 @default.
• W2001032368 hasConcept C2776109719 @default.
• W2001032368 hasConcept C2777167505 @default.
• W2001032368 hasConcept C2777476445 @default.
• W2001032368 hasConcept C2777497464 @default.
• W2001032368 hasConcept C2778592357 @default.
• W2001032368 hasConcept C2780115381 @default.
• W2001032368 hasConcept C2781159505 @default.
• W2001032368 hasConcept C28406088 @default.
• W2001032368 hasConcept C538909803 @default.
• W2001032368 hasConcept C55493867 @default.
• W2001032368 hasConcept C79879829 @default.
• W2001032368 hasConcept C86803240 @default.
• W2001032368 hasConceptScore W2001032368C153911025 @default.
• W2001032368 hasConceptScore W2001032368C181199279 @default.
• W2001032368 hasConceptScore W2001032368C185592680 @default.
• W2001032368 hasConceptScore W2001032368C2776109719 @default.
• W2001032368 hasConceptScore W2001032368C2777167505 @default.
• W2001032368 hasConceptScore W2001032368C2777476445 @default.
• W2001032368 hasConceptScore W2001032368C2777497464 @default.
• W2001032368 hasConceptScore W2001032368C2778592357 @default.
• W2001032368 hasConceptScore W2001032368C2780115381 @default.
• W2001032368 hasConceptScore W2001032368C2781159505 @default.
• W2001032368 hasConceptScore W2001032368C28406088 @default.
• W2001032368 hasConceptScore W2001032368C538909803 @default.
• W2001032368 hasConceptScore W2001032368C55493867 @default.
• W2001032368 hasConceptScore W2001032368C79879829 @default.
• W2001032368 hasConceptScore W2001032368C86803240 @default.
• W2001032368 hasIssue "7" @default.
• W2001032368 hasLocation W20010323681 @default.
• W2001032368 hasLocation W20010323682 @default.
• W2001032368 hasOpenAccess W2001032368 @default.
• W2001032368 hasPrimaryLocation W20010323681 @default.
• W2001032368 hasRelatedWork W1868357828 @default.
• W2001032368 hasRelatedWork W1980119632 @default.
• W2001032368 hasRelatedWork W2001032368 @default.
• W2001032368 hasRelatedWork W2018981379 @default.
• W2001032368 hasRelatedWork W2053560868 @default.
• W2001032368 hasRelatedWork W2056086472 @default.
• W2001032368 hasRelatedWork W2078471843 @default.
• W2001032368 hasRelatedWork W2082211825 @default.
• W2001032368 hasRelatedWork W2142511487 @default.
• W2001032368 hasRelatedWork W2273940923 @default.
• W2001032368 hasVolume "50" @default.
• W2001032368 isParatext "false" @default.
• W2001032368 isRetracted "false" @default.
• W2001032368 magId "2001032368" @default.
• W2001032368 workType "article" @default.