FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001036451> ?p ?o ?g. }

• W2001036451 endingPage "369" @default.
• W2001036451 startingPage "361" @default.
• W2001036451 abstract "Summary Background Basophils are increasingly recognized as playing important roles in the immune responses of allergic diseases and helminth infections. One of the main obstacles to studying basophils has been the lack of a simple and rapid assay to measure basophil activation in mice. Objective The purpose of this study was to develop an assay to measure murine basophil activation. Methods Mouse blood cells were stained with various combinations of positive and negative markers for basophils – sorted and then assessed for basophil purity by May‐Grünwald staining of cytospins. Once a flow cytometric strategy for staining basophils was determined, basophil surface expression of CD200R was assessed by multi‐colour flow cytometry after stimulation of whole blood with anti‐IgE, ionomycin or N‐formyl MetLeuPhe (fMLP). Confirmation of basophil activation was assessed by concomitant staining of cells for intracellular IL‐4. To test the ability of flow cytometric basophil CD200R measurements to assess for antigen‐specific IgE‐mediated activation of basophils, surface CD200R expression in response to in vitro stimulation with media alone, helminth antigen or ovalbumin was measured on basophils obtained from control mice, mice infected with helminths and mice sensitized to ovalbumin. Results Using anti‐IgE‐FITC as a positive marker and a combination of anti‐CD4‐PERCP and anti‐B220‐PERCP as negative markers resulted in a well‐separated basophil population. Additional staining with anti‐CD200R‐PE demonstrated that (1) basophil CD200R expression increases in response to anti‐IgE, ionomycin and fMLP, (2) most CD200R‐positive basophils also stain positively for IL‐4 and (3) CD200R expression increases after antigen‐specific activation of basophils in murine models of helminth disease and allergy. Conclusion We developed a multi‐colour flow cytometry assay that measures murine basophil activation by utilizing CD200R as an activation marker. This assay is straightforward and rapid, taking approximately half a day for obtaining blood, in vitro stimulation and flow cytometric analysis." @default.
• W2001036451 created "2016-06-24" @default.
• W2001036451 creator A5020458885 @default.
• W2001036451 creator A5026752464 @default.
• W2001036451 creator A5033576692 @default.
• W2001036451 creator A5042049581 @default.
• W2001036451 date "2009-01-28" @default.
• W2001036451 modified "2023-10-16" @default.
• W2001036451 title "CD200R surface expression as a marker of murine basophil activation" @default.
• W2001036451 cites W1512243818 @default.
• W2001036451 cites W1566199883 @default.
• W2001036451 cites W1571985114 @default.
• W2001036451 cites W1905186867 @default.
• W2001036451 cites W2022017784 @default.
• W2001036451 cites W2028022848 @default.
• W2001036451 cites W2036567725 @default.
• W2001036451 cites W2047601088 @default.
• W2001036451 cites W2051587095 @default.
• W2001036451 cites W2061701719 @default.
• W2001036451 cites W2063329465 @default.
• W2001036451 cites W2066531844 @default.
• W2001036451 cites W2074287771 @default.
• W2001036451 cites W2106634168 @default.
• W2001036451 cites W2113844670 @default.
• W2001036451 cites W2119814069 @default.
• W2001036451 cites W2123760021 @default.
• W2001036451 cites W2131966414 @default.
• W2001036451 cites W2159744102 @default.
• W2001036451 doi "https://doi.org/10.1111/j.1365-2222.2008.03154.x" @default.
• W2001036451 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2743132" @default.
• W2001036451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19134017" @default.
• W2001036451 hasPublicationYear "2009" @default.
• W2001036451 type Work @default.
• W2001036451 sameAs 2001036451 @default.
• W2001036451 citedByCount "48" @default.
• W2001036451 countsByYear W20010364512012 @default.
• W2001036451 countsByYear W20010364512013 @default.
• W2001036451 countsByYear W20010364512014 @default.
• W2001036451 countsByYear W20010364512015 @default.
• W2001036451 countsByYear W20010364512016 @default.
• W2001036451 countsByYear W20010364512017 @default.
• W2001036451 countsByYear W20010364512018 @default.
• W2001036451 countsByYear W20010364512019 @default.
• W2001036451 countsByYear W20010364512020 @default.
• W2001036451 countsByYear W20010364512021 @default.
• W2001036451 countsByYear W20010364512022 @default.
• W2001036451 countsByYear W20010364512023 @default.
• W2001036451 crossrefType "journal-article" @default.
• W2001036451 hasAuthorship W2001036451A5020458885 @default.
• W2001036451 hasAuthorship W2001036451A5026752464 @default.
• W2001036451 hasAuthorship W2001036451A5033576692 @default.
• W2001036451 hasAuthorship W2001036451A5042049581 @default.
• W2001036451 hasBestOaLocation W20010364511 @default.
• W2001036451 hasConcept C141105273 @default.
• W2001036451 hasConcept C147483822 @default.
• W2001036451 hasConcept C153911025 @default.
• W2001036451 hasConcept C159654299 @default.
• W2001036451 hasConcept C203014093 @default.
• W2001036451 hasConcept C2776946954 @default.
• W2001036451 hasConcept C2776989580 @default.
• W2001036451 hasConcept C2777456892 @default.
• W2001036451 hasConcept C2779176990 @default.
• W2001036451 hasConcept C2908647359 @default.
• W2001036451 hasConcept C553184892 @default.
• W2001036451 hasConcept C71924100 @default.
• W2001036451 hasConcept C79879829 @default.
• W2001036451 hasConcept C86803240 @default.
• W2001036451 hasConcept C95444343 @default.
• W2001036451 hasConcept C99454951 @default.
• W2001036451 hasConceptScore W2001036451C141105273 @default.
• W2001036451 hasConceptScore W2001036451C147483822 @default.
• W2001036451 hasConceptScore W2001036451C153911025 @default.
• W2001036451 hasConceptScore W2001036451C159654299 @default.
• W2001036451 hasConceptScore W2001036451C203014093 @default.
• W2001036451 hasConceptScore W2001036451C2776946954 @default.
• W2001036451 hasConceptScore W2001036451C2776989580 @default.
• W2001036451 hasConceptScore W2001036451C2777456892 @default.
• W2001036451 hasConceptScore W2001036451C2779176990 @default.
• W2001036451 hasConceptScore W2001036451C2908647359 @default.
• W2001036451 hasConceptScore W2001036451C553184892 @default.
• W2001036451 hasConceptScore W2001036451C71924100 @default.
• W2001036451 hasConceptScore W2001036451C79879829 @default.
• W2001036451 hasConceptScore W2001036451C86803240 @default.
• W2001036451 hasConceptScore W2001036451C95444343 @default.
• W2001036451 hasConceptScore W2001036451C99454951 @default.
• W2001036451 hasIssue "3" @default.
• W2001036451 hasLocation W20010364511 @default.
• W2001036451 hasLocation W20010364512 @default.
• W2001036451 hasLocation W20010364513 @default.
• W2001036451 hasLocation W20010364514 @default.
• W2001036451 hasOpenAccess W2001036451 @default.
• W2001036451 hasPrimaryLocation W20010364511 @default.
• W2001036451 hasRelatedWork W134701817 @default.
• W2001036451 hasRelatedWork W1611533949 @default.
• W2001036451 hasRelatedWork W1941449931 @default.
• W2001036451 hasRelatedWork W1978963588 @default.
• W2001036451 hasRelatedWork W2012769899 @default.
• W2001036451 hasRelatedWork W2094810816 @default.

• next