Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001051266> ?p ?o ?g. }
- W2001051266 endingPage "1131" @default.
- W2001051266 startingPage "1115" @default.
- W2001051266 abstract "Several transcription factors (TFs) coordinate to regulate expression of specific genes at the transcriptional level. In Arabidopsis thaliana it is estimated that approximately 10% of all genes encode TFs or TF-like proteins. It is important to identify target genes that are directly regulated by TFs in order to understand the complete picture of a plant’s transcriptome profile. Here, we investigate the role of the LONG HYPOCOTYL5 (HY5) transcription factor that acts as a regulator of photomorphogenesis. We used an in vitro genomic DNA binding assay coupled with immunoprecipitation and next-generation sequencing (gDB-seq) instead of the in vivo chromatin immunoprecipitation (ChIP)-based methods. The results demonstrate that the HY5-binding motif predicted here was similar to the motif reported previously and that in vitro HY5-binding loci largely overlapped with the HY5-targeted candidate genes identified in previous ChIP-chip analysis. By combining these results with microarray analysis, we identified hundreds of HY5-binding genes that were differentially expressed in hy5. We also observed delayed induction of some transcripts of HY5-binding genes in hy5 mutants in response to blue-light exposure after dark treatment. Thus, an in vitro gDNA-binding assay coupled with sequencing is a convenient and powerful method to bridge the gap between identifying TF binding potential and establishing function." @default.
- W2001051266 created "2016-06-24" @default.
- W2001051266 creator A5003729142 @default.
- W2001051266 creator A5034218421 @default.
- W2001051266 creator A5038514369 @default.
- W2001051266 creator A5059614146 @default.
- W2001051266 creator A5066711822 @default.
- W2001051266 creator A5079242442 @default.
- W2001051266 date "2014-12-19" @default.
- W2001051266 modified "2023-09-27" @default.
- W2001051266 title "Next-Generation Sequencing of Genomic DNA Fragments Bound to a Transcription Factor in Vitro Reveals Its Regulatory Potential" @default.
- W2001051266 cites W1665182114 @default.
- W2001051266 cites W1964733653 @default.
- W2001051266 cites W2014494440 @default.
- W2001051266 cites W2015246122 @default.
- W2001051266 cites W2015266107 @default.
- W2001051266 cites W2024058212 @default.
- W2001051266 cites W2026690931 @default.
- W2001051266 cites W2028189102 @default.
- W2001051266 cites W2033169664 @default.
- W2001051266 cites W2033667297 @default.
- W2001051266 cites W2044536026 @default.
- W2001051266 cites W2057533559 @default.
- W2001051266 cites W2081277704 @default.
- W2001051266 cites W2084219391 @default.
- W2001051266 cites W2097065948 @default.
- W2001051266 cites W2098664089 @default.
- W2001051266 cites W2102566745 @default.
- W2001051266 cites W2109202455 @default.
- W2001051266 cites W2112713711 @default.
- W2001051266 cites W2115431543 @default.
- W2001051266 cites W2118299395 @default.
- W2001051266 cites W2124753150 @default.
- W2001051266 cites W2130472562 @default.
- W2001051266 cites W2133009404 @default.
- W2001051266 cites W2139008831 @default.
- W2001051266 cites W2139514307 @default.
- W2001051266 cites W2140918488 @default.
- W2001051266 cites W2141458291 @default.
- W2001051266 cites W2148711195 @default.
- W2001051266 cites W2152121035 @default.
- W2001051266 cites W2152324471 @default.
- W2001051266 cites W2152338515 @default.
- W2001051266 cites W2153286183 @default.
- W2001051266 cites W2156231007 @default.
- W2001051266 cites W2165221134 @default.
- W2001051266 cites W2170551349 @default.
- W2001051266 cites W2170664592 @default.
- W2001051266 cites W2171808845 @default.
- W2001051266 doi "https://doi.org/10.3390/genes5041115" @default.
- W2001051266 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4276929" @default.
- W2001051266 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25534860" @default.
- W2001051266 hasPublicationYear "2014" @default.
- W2001051266 type Work @default.
- W2001051266 sameAs 2001051266 @default.
- W2001051266 citedByCount "18" @default.
- W2001051266 countsByYear W20010512662015 @default.
- W2001051266 countsByYear W20010512662016 @default.
- W2001051266 countsByYear W20010512662017 @default.
- W2001051266 countsByYear W20010512662018 @default.
- W2001051266 countsByYear W20010512662019 @default.
- W2001051266 countsByYear W20010512662020 @default.
- W2001051266 countsByYear W20010512662021 @default.
- W2001051266 crossrefType "journal-article" @default.
- W2001051266 hasAuthorship W2001051266A5003729142 @default.
- W2001051266 hasAuthorship W2001051266A5034218421 @default.
- W2001051266 hasAuthorship W2001051266A5038514369 @default.
- W2001051266 hasAuthorship W2001051266A5059614146 @default.
- W2001051266 hasAuthorship W2001051266A5066711822 @default.
- W2001051266 hasAuthorship W2001051266A5079242442 @default.
- W2001051266 hasBestOaLocation W20010512661 @default.
- W2001051266 hasConcept C101762097 @default.
- W2001051266 hasConcept C104317684 @default.
- W2001051266 hasConcept C134320426 @default.
- W2001051266 hasConcept C143065580 @default.
- W2001051266 hasConcept C150194340 @default.
- W2001051266 hasConcept C162317418 @default.
- W2001051266 hasConcept C167227067 @default.
- W2001051266 hasConcept C176619506 @default.
- W2001051266 hasConcept C2779491563 @default.
- W2001051266 hasConcept C3662595 @default.
- W2001051266 hasConcept C54355233 @default.
- W2001051266 hasConcept C70721500 @default.
- W2001051266 hasConcept C71829478 @default.
- W2001051266 hasConcept C83640560 @default.
- W2001051266 hasConcept C8415881 @default.
- W2001051266 hasConcept C86339819 @default.
- W2001051266 hasConcept C86803240 @default.
- W2001051266 hasConcept C95371953 @default.
- W2001051266 hasConceptScore W2001051266C101762097 @default.
- W2001051266 hasConceptScore W2001051266C104317684 @default.
- W2001051266 hasConceptScore W2001051266C134320426 @default.
- W2001051266 hasConceptScore W2001051266C143065580 @default.
- W2001051266 hasConceptScore W2001051266C150194340 @default.
- W2001051266 hasConceptScore W2001051266C162317418 @default.
- W2001051266 hasConceptScore W2001051266C167227067 @default.
- W2001051266 hasConceptScore W2001051266C176619506 @default.
- W2001051266 hasConceptScore W2001051266C2779491563 @default.