FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001059775> ?p ?o ?g. }

• W2001059775 endingPage "601" @default.
• W2001059775 startingPage "591" @default.
• W2001059775 abstract "A metabolic reaction network is developed for the estimation of the stoichiometric production of adenosine triphosphate (ATP) in animal cell culture. By using the material balance data from fed-batch and batch cultures of hybridoma cells, the stoichiometric ATP productions are determined with estimated effective P/O ratios of 2 for NADH and 1.2 for FADH2. A significant percentage of the ATP requirement (16–41%) in hybridoma cells is generated directly from free energy release without the participation of oxygen. The oxidative phosphorylation of NADH accounts for about 60% of the total ATP production in the fed-batch cultures and about 47% in the batch culture. The oxidative phosphorylation of FADH2 accounts for less then 20% of the total ATP production in all cases. A fractional model is devised to analyze the contribution of each nutrient to the ATP production. Results show that a majority of the ATP is produced from glucose metabolism (60–76%). Less than 30% of the ATP is derived from glutamine, and less than 11% is derived from other essential amino acids. The analysis also shows that the glycolytic pathway generates more ATP in the batch (41%) than in the fed-batch (<27%) cultures. The TCA cycle provides 51–68% of the total ATP production. The calculated stoichiometric oxygen consumption differs among the batch and fed-batch cultures, depending on the glucose concentration. This result suggests that the relationship between the oxygen uptake rate (OUR) and cell growth may change with the culture conditions. However, the calculated respiratory quotient (RQ) is relatively constant in all cases. A linear relationship is obtained between the specific ATP production rate and the specific cell growth rate. The maximum ATP yield and the maintenance ATP requirement are determined based on this linear relationship. The biosynthetic ATP demand estimated from the dry cell weight and cell composition is significantly lower than that calculated from the maximum ATP yield, indicating that the non-growth-associated ATP demand may contain other factors than what is considered in the estimation of the biosynthetic ATP demand. © 1996 John Wiley & Sons, Inc." @default.
• W2001059775 created "2016-06-24" @default.
• W2001059775 creator A5044808093 @default.
• W2001059775 creator A5079189759 @default.
• W2001059775 date "2000-03-26" @default.
• W2001059775 modified "2023-10-10" @default.
• W2001059775 title "Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network" @default.
• W2001059775 cites W138314035 @default.
• W2001059775 cites W1490544414 @default.
• W2001059775 cites W1567637492 @default.
• W2001059775 cites W1597238891 @default.
• W2001059775 cites W1761660438 @default.
• W2001059775 cites W1973847153 @default.
• W2001059775 cites W1976269906 @default.
• W2001059775 cites W2002172128 @default.
• W2001059775 cites W2009326360 @default.
• W2001059775 cites W2013859827 @default.
• W2001059775 cites W2018008917 @default.
• W2001059775 cites W2025138920 @default.
• W2001059775 cites W2026161384 @default.
• W2001059775 cites W2033052885 @default.
• W2001059775 cites W2040603216 @default.
• W2001059775 cites W2044782966 @default.
• W2001059775 cites W2049814691 @default.
• W2001059775 cites W2051954404 @default.
• W2001059775 cites W2054158218 @default.
• W2001059775 cites W2066231536 @default.
• W2001059775 cites W2076313026 @default.
• W2001059775 cites W2083198296 @default.
• W2001059775 cites W2095951127 @default.
• W2001059775 cites W2107792766 @default.
• W2001059775 cites W2121287972 @default.
• W2001059775 cites W2141783351 @default.
• W2001059775 cites W2162644786 @default.
• W2001059775 cites W2166242327 @default.
• W2001059775 cites W2166355731 @default.
• W2001059775 cites W2897745919 @default.
• W2001059775 cites W4241281124 @default.
• W2001059775 cites W4246052834 @default.
• W2001059775 doi "https://doi.org/10.1002/(sici)1097-0290(19961205)52:5<591::aid-bit6>3.0.co;2-e" @default.
• W2001059775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18629932" @default.
• W2001059775 hasPublicationYear "2000" @default.
• W2001059775 type Work @default.
• W2001059775 sameAs 2001059775 @default.
• W2001059775 citedByCount "58" @default.
• W2001059775 countsByYear W20010597752012 @default.
• W2001059775 countsByYear W20010597752013 @default.
• W2001059775 countsByYear W20010597752014 @default.
• W2001059775 countsByYear W20010597752016 @default.
• W2001059775 countsByYear W20010597752017 @default.
• W2001059775 countsByYear W20010597752018 @default.
• W2001059775 countsByYear W20010597752019 @default.
• W2001059775 countsByYear W20010597752020 @default.
• W2001059775 countsByYear W20010597752021 @default.
• W2001059775 countsByYear W20010597752022 @default.
• W2001059775 crossrefType "journal-article" @default.
• W2001059775 hasAuthorship W2001059775A5044808093 @default.
• W2001059775 hasAuthorship W2001059775A5079189759 @default.
• W2001059775 hasConcept C144082473 @default.
• W2001059775 hasConcept C178790620 @default.
• W2001059775 hasConcept C185592680 @default.
• W2001059775 hasConcept C20251656 @default.
• W2001059775 hasConcept C2779349466 @default.
• W2001059775 hasConcept C2779564974 @default.
• W2001059775 hasConcept C515207424 @default.
• W2001059775 hasConcept C540031477 @default.
• W2001059775 hasConcept C55493867 @default.
• W2001059775 hasConcept C57600042 @default.
• W2001059775 hasConcept C62231903 @default.
• W2001059775 hasConcept C86803240 @default.
• W2001059775 hasConcept C98488077 @default.
• W2001059775 hasConceptScore W2001059775C144082473 @default.
• W2001059775 hasConceptScore W2001059775C178790620 @default.
• W2001059775 hasConceptScore W2001059775C185592680 @default.
• W2001059775 hasConceptScore W2001059775C20251656 @default.
• W2001059775 hasConceptScore W2001059775C2779349466 @default.
• W2001059775 hasConceptScore W2001059775C2779564974 @default.
• W2001059775 hasConceptScore W2001059775C515207424 @default.
• W2001059775 hasConceptScore W2001059775C540031477 @default.
• W2001059775 hasConceptScore W2001059775C55493867 @default.
• W2001059775 hasConceptScore W2001059775C57600042 @default.
• W2001059775 hasConceptScore W2001059775C62231903 @default.
• W2001059775 hasConceptScore W2001059775C86803240 @default.
• W2001059775 hasConceptScore W2001059775C98488077 @default.
• W2001059775 hasIssue "5" @default.
• W2001059775 hasLocation W20010597751 @default.
• W2001059775 hasLocation W20010597752 @default.
• W2001059775 hasOpenAccess W2001059775 @default.
• W2001059775 hasPrimaryLocation W20010597751 @default.
• W2001059775 hasRelatedWork W1866222897 @default.
• W2001059775 hasRelatedWork W2016733795 @default.
• W2001059775 hasRelatedWork W2029624447 @default.
• W2001059775 hasRelatedWork W2071106723 @default.
• W2001059775 hasRelatedWork W2081780014 @default.
• W2001059775 hasRelatedWork W2094643509 @default.
• W2001059775 hasRelatedWork W2111784969 @default.
• W2001059775 hasRelatedWork W2119738915 @default.
• W2001059775 hasRelatedWork W2142457100 @default.

• next