Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001065366> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2001065366 endingPage "76" @default.
- W2001065366 startingPage "69" @default.
- W2001065366 abstract "In this review we discuss our progress in investigating the pro tease which is responsible for generating des (1-3) insulin-like growth factor-I (IGF-I), a more potent form of IGF-I devoid of the first 3 N-terminal amino acid residues. Characterization of the protease activity indicated that it was most likely to be a member of the serine protease family. Although some activity was detected at neutral pH, the activity was optimal at pH 5.5. The enzyme was purified from rat liver utilizing ion-exchange, chromatofocusing, phenylsepharose chromatography followed by high performance liquid chematography (HPLC). A single band of - 31 kD was observed on silver stained sodium dodecyl sulphate-polyachylamide gel electrophoresis (SDS-PAGE) gels. We developed a rapid assay to quantify this protease activity in serum and tissue extracts. The activity was detected in mouse, rat, human serum and rat tissue extracts. The level of protease activity in the tissue extracts demonstrated the following order: liver>testes>heart>skeletal muscle>lung>thymus>kidney>brain>spleen. Protease activity was significantly higher in sera from hypophysectomized rats than in intact rats and growth hormone (GH) replacement reduced enzyme activity. In addition, serum protease activity was significantly elevated in diabetic rats and correlated with serum glucose concentrations. These data demonstrate that the proteolytic conversion of IGF-I to a more active form is enhanced under conditions associated with low IGF-I, insulin and GH levels and suggest that this conversion may be an additional site of regulation of IGF-I action." @default.
- W2001065366 created "2016-06-24" @default.
- W2001065366 creator A5009993998 @default.
- W2001065366 creator A5014997892 @default.
- W2001065366 creator A5073592675 @default.
- W2001065366 date "1996-01-01" @default.
- W2001065366 modified "2023-10-16" @default.
- W2001065366 title "Proteolytic Conversion of Insulin-like Growth Factor-I to des (1-3) Insulin-like Growth Factor-I: A Further Site of Regulation of Insulin-like Growth Factor-I Action" @default.
- W2001065366 cites W1548258669 @default.
- W2001065366 cites W1556893107 @default.
- W2001065366 cites W1876185986 @default.
- W2001065366 cites W1964113315 @default.
- W2001065366 cites W1984830318 @default.
- W2001065366 cites W2011479978 @default.
- W2001065366 cites W2028527312 @default.
- W2001065366 cites W2029114578 @default.
- W2001065366 cites W2071001517 @default.
- W2001065366 cites W2090025926 @default.
- W2001065366 cites W2093196719 @default.
- W2001065366 cites W2163969205 @default.
- W2001065366 cites W2176506025 @default.
- W2001065366 cites W98280979 @default.
- W2001065366 doi "https://doi.org/10.1297/cpe.5.supple8_69" @default.
- W2001065366 hasPublicationYear "1996" @default.
- W2001065366 type Work @default.
- W2001065366 sameAs 2001065366 @default.
- W2001065366 citedByCount "0" @default.
- W2001065366 crossrefType "journal-article" @default.
- W2001065366 hasAuthorship W2001065366A5009993998 @default.
- W2001065366 hasAuthorship W2001065366A5014997892 @default.
- W2001065366 hasAuthorship W2001065366A5073592675 @default.
- W2001065366 hasBestOaLocation W20010653661 @default.
- W2001065366 hasConcept C126322002 @default.
- W2001065366 hasConcept C134018914 @default.
- W2001065366 hasConcept C170493617 @default.
- W2001065366 hasConcept C181199279 @default.
- W2001065366 hasConcept C182220744 @default.
- W2001065366 hasConcept C2775960820 @default.
- W2001065366 hasConcept C2776714187 @default.
- W2001065366 hasConcept C2776829962 @default.
- W2001065366 hasConcept C2777807008 @default.
- W2001065366 hasConcept C2779306644 @default.
- W2001065366 hasConcept C2780091579 @default.
- W2001065366 hasConcept C2780689927 @default.
- W2001065366 hasConcept C55493867 @default.
- W2001065366 hasConcept C71924100 @default.
- W2001065366 hasConcept C86803240 @default.
- W2001065366 hasConcept C96294017 @default.
- W2001065366 hasConceptScore W2001065366C126322002 @default.
- W2001065366 hasConceptScore W2001065366C134018914 @default.
- W2001065366 hasConceptScore W2001065366C170493617 @default.
- W2001065366 hasConceptScore W2001065366C181199279 @default.
- W2001065366 hasConceptScore W2001065366C182220744 @default.
- W2001065366 hasConceptScore W2001065366C2775960820 @default.
- W2001065366 hasConceptScore W2001065366C2776714187 @default.
- W2001065366 hasConceptScore W2001065366C2776829962 @default.
- W2001065366 hasConceptScore W2001065366C2777807008 @default.
- W2001065366 hasConceptScore W2001065366C2779306644 @default.
- W2001065366 hasConceptScore W2001065366C2780091579 @default.
- W2001065366 hasConceptScore W2001065366C2780689927 @default.
- W2001065366 hasConceptScore W2001065366C55493867 @default.
- W2001065366 hasConceptScore W2001065366C71924100 @default.
- W2001065366 hasConceptScore W2001065366C86803240 @default.
- W2001065366 hasConceptScore W2001065366C96294017 @default.
- W2001065366 hasIssue "Supple8" @default.
- W2001065366 hasLocation W20010653661 @default.
- W2001065366 hasOpenAccess W2001065366 @default.
- W2001065366 hasPrimaryLocation W20010653661 @default.
- W2001065366 hasRelatedWork W1975650642 @default.
- W2001065366 hasRelatedWork W2019149333 @default.
- W2001065366 hasRelatedWork W2042033030 @default.
- W2001065366 hasRelatedWork W2044988659 @default.
- W2001065366 hasRelatedWork W2060694919 @default.
- W2001065366 hasRelatedWork W2098742882 @default.
- W2001065366 hasRelatedWork W2491451478 @default.
- W2001065366 hasRelatedWork W4236502113 @default.
- W2001065366 hasRelatedWork W4250536708 @default.
- W2001065366 hasRelatedWork W4251636735 @default.
- W2001065366 hasVolume "5" @default.
- W2001065366 isParatext "false" @default.
- W2001065366 isRetracted "false" @default.
- W2001065366 magId "2001065366" @default.
- W2001065366 workType "article" @default.