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- W2001100139 abstract "Alditols were determined in aqueous solution using a novel indirect photometric technique as a post-column detection method. It allowed the use of the common UV detector for these transparent analytes. The method is based on the decrease of absorbance (λ = 347 nm) due to the competitive complexation of molybdate by the chloranilate ion (coloured complex) and an alditol (colourless complex). The experimental conditions were defined in a preliminary study which related the sensitivity for each analyte to the formation constant of its molybdate complex. d-Glucose, which forms a weak complex, does not interfere. The method was applied to the HPLC determination of mixtures of alditols with separation on a calcium column. The calibration curves are linear in the concentration range 0–100 mg·1−1. It demonstrates that indirect photometric detection may be used for the selective determination of specified analytes, in contrast with its usual application as a universal detection technique." @default.
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- W2001100139 date "1995-01-01" @default.
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- W2001100139 title "High-performance liquid chromatography of alditols with indirect photometric detection" @default.
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- W2001100139 doi "https://doi.org/10.1016/0021-9673(94)00981-e" @default.
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