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- W2001170040 abstract "Reporter genes are the tools of ever-increasing importance in the study of yeast gene expression. Their versatility and the ease of use have made them widely available and ongoing developments will undoubtedly continue to increase their usefulness and their potential applications. A few examples of studies on transcriptional, translational, and post-translational regulation of gene expression are used in this chapter to illustrate some general principles of the use of reporter genes. Many of the reporters developed for use in yeast were originally designed to investigate promoter activity. A good example for this is the original description of the chloramphenicol acetyltransferase (CAT) assay in yeast. The authors of this study demonstrated the validity of CAT as a yeast reporter gene by constructing vectors that contained the coding region of the bacterial cat gene, but no promoter. Any essential gene can be used as a reporter in the corresponding knockout yeast strains because in these strains the ability to grow depends on the expression of the respective gene. The growth rate, thus, provides an easy readout for the expression of the gene." @default.
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- W2001170040 date "2007-01-01" @default.
- W2001170040 modified "2023-10-08" @default.
- W2001170040 title "8 Reporter Genes and Their Uses in Studying Yeast Gene Expression" @default.
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- W2001170040 doi "https://doi.org/10.1016/s0580-9517(06)36008-4" @default.
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