FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001179863> ?p ?o ?g. }

• W2001179863 endingPage "190" @default.
• W2001179863 startingPage "185" @default.
• W2001179863 abstract "The mouse lymphoma assay (MLA) is the most widely used in vitro mammalian gene mutation assay. It detects various mutation events involving the thymidine kinase (Tk) gene in L5178Y/Tk+/− −3.7.2C mouse lymphoma cells. Mutants are detected using a thymidine analogue that arrests the growth of cells containing a functional Tk gene. However, there are a number of potential test chemicals that are thymidine analogues, and there is a problem when using the MLA to evaluate the mutagenicity of these chemicals. Thymidine analogues are activated by Tk before eliciting their toxicity. Therefore, any pre-existing Tk−/− mutants may avoid the toxicity of the test chemical and obtain a growth advantage over the Tk+/− cells, increasing the Tk mutant frequency (MF) in the culture via a selection mechanism. This potential mutant selection effect needs to be distinguished from de novo mutant induction in order to properly evaluate the mutagenicity of these chemicals. Here we describe a simple MLA study design that can differentiate between the selection of pre-existing mutants and de novo mutant induction. Trifluorothymidine (TFT), a thymidine analogue and the selection agent normally used in the MLA, and 4-nitroquinoline-1-oxide (4-NQO), a potent mutagen, were used to treat cells from two different Tk+/− mouse lymphoma cell cultures with different background MFs (approximately 112 and 305 × 10−6). Both agents significantly increased the Tk MFs in both the normal and high background cultures (p < 0.01). In 4-NQO-treated cultures, the induced MFs (MF of treated culture − MF of control) for the cultures with different background MFs were about the same (p > 0.1), while in TFT-treated cultures, they were significantly different (p < 0.01). In TFT-treated cultures, the fold-increases of MF (MF of treated culture/MF of control) for the cultures with different background MFs were about the same (p > 0.1), while in 4-NQO-treated cultures, they were significantly different (p < 0.01). This study confirms that, when de novo mutations are induced, the induced MF is the same for cultures with normal and artificially high background MFs. In situations where the increase in MF is due solely to selection of pre-existing mutants, the “induced” MF will be a multiple of the background MF and the magnitude of the increase of the induced MF will depend upon the magnitude of the background MF. Our results demonstrate that it is possible, using this experimental design, to distinguish between chemicals acting primarily via the selection of pre-existing Tk mutants and those inducing de novo mutants in the MLA." @default.
• W2001179863 created "2016-06-24" @default.
• W2001179863 creator A5015628950 @default.
• W2001179863 creator A5035119519 @default.
• W2001179863 creator A5078091393 @default.
• W2001179863 date "2007-01-01" @default.
• W2001179863 modified "2023-09-28" @default.
• W2001179863 title "A method to distinguish between the de novo induction of thymidine kinase mutants and the selection of pre-existing thymidine kinase mutants in the mouse lymphoma assay" @default.
• W2001179863 cites W1973454901 @default.
• W2001179863 cites W1975827721 @default.
• W2001179863 cites W1982992257 @default.
• W2001179863 cites W1988562694 @default.
• W2001179863 cites W1996964294 @default.
• W2001179863 cites W2008054246 @default.
• W2001179863 cites W2008812480 @default.
• W2001179863 cites W2031939955 @default.
• W2001179863 cites W2035972738 @default.
• W2001179863 cites W2036923252 @default.
• W2001179863 cites W2044877337 @default.
• W2001179863 cites W2081619063 @default.
• W2001179863 cites W2084243348 @default.
• W2001179863 cites W2085340263 @default.
• W2001179863 cites W2105029470 @default.
• W2001179863 cites W2125974014 @default.
• W2001179863 cites W2129873851 @default.
• W2001179863 cites W2140638516 @default.
• W2001179863 cites W2207298758 @default.
• W2001179863 cites W2219671671 @default.
• W2001179863 cites W2253351494 @default.
• W2001179863 cites W33919623 @default.
• W2001179863 cites W354886346 @default.
• W2001179863 cites W593287483 @default.
• W2001179863 doi "https://doi.org/10.1016/j.mrgentox.2006.09.002" @default.
• W2001179863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17055776" @default.
• W2001179863 hasPublicationYear "2007" @default.
• W2001179863 type Work @default.
• W2001179863 sameAs 2001179863 @default.
• W2001179863 citedByCount "2" @default.
• W2001179863 crossrefType "journal-article" @default.
• W2001179863 hasAuthorship W2001179863A5015628950 @default.
• W2001179863 hasAuthorship W2001179863A5035119519 @default.
• W2001179863 hasAuthorship W2001179863A5078091393 @default.
• W2001179863 hasConcept C104317684 @default.
• W2001179863 hasConcept C143065580 @default.
• W2001179863 hasConcept C153911025 @default.
• W2001179863 hasConcept C202751555 @default.
• W2001179863 hasConcept C2522874641 @default.
• W2001179863 hasConcept C2776147627 @default.
• W2001179863 hasConcept C2776639595 @default.
• W2001179863 hasConcept C2777108182 @default.
• W2001179863 hasConcept C2781196997 @default.
• W2001179863 hasConcept C501734568 @default.
• W2001179863 hasConcept C54355233 @default.
• W2001179863 hasConcept C552990157 @default.
• W2001179863 hasConcept C55493867 @default.
• W2001179863 hasConcept C81885089 @default.
• W2001179863 hasConcept C86803240 @default.
• W2001179863 hasConceptScore W2001179863C104317684 @default.
• W2001179863 hasConceptScore W2001179863C143065580 @default.
• W2001179863 hasConceptScore W2001179863C153911025 @default.
• W2001179863 hasConceptScore W2001179863C202751555 @default.
• W2001179863 hasConceptScore W2001179863C2522874641 @default.
• W2001179863 hasConceptScore W2001179863C2776147627 @default.
• W2001179863 hasConceptScore W2001179863C2776639595 @default.
• W2001179863 hasConceptScore W2001179863C2777108182 @default.
• W2001179863 hasConceptScore W2001179863C2781196997 @default.
• W2001179863 hasConceptScore W2001179863C501734568 @default.
• W2001179863 hasConceptScore W2001179863C54355233 @default.
• W2001179863 hasConceptScore W2001179863C552990157 @default.
• W2001179863 hasConceptScore W2001179863C55493867 @default.
• W2001179863 hasConceptScore W2001179863C81885089 @default.
• W2001179863 hasConceptScore W2001179863C86803240 @default.
• W2001179863 hasIssue "1-2" @default.
• W2001179863 hasLocation W20011798631 @default.
• W2001179863 hasLocation W20011798632 @default.
• W2001179863 hasOpenAccess W2001179863 @default.
• W2001179863 hasPrimaryLocation W20011798631 @default.
• W2001179863 hasRelatedWork W1897859244 @default.
• W2001179863 hasRelatedWork W2023301897 @default.
• W2001179863 hasRelatedWork W2024850895 @default.
• W2001179863 hasRelatedWork W2034290380 @default.
• W2001179863 hasRelatedWork W2050627573 @default.
• W2001179863 hasRelatedWork W2059929579 @default.
• W2001179863 hasRelatedWork W2094451675 @default.
• W2001179863 hasRelatedWork W2147466517 @default.
• W2001179863 hasRelatedWork W2171728434 @default.
• W2001179863 hasRelatedWork W4239997277 @default.
• W2001179863 hasVolume "626" @default.
• W2001179863 isParatext "false" @default.
• W2001179863 isRetracted "false" @default.
• W2001179863 magId "2001179863" @default.
• W2001179863 workType "article" @default.