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• W2001199176 abstract "The conversion of dihydroquercetin to catechin and procyanidin was studied in maturing wild type barley (Hordeum vulgare L., cv. Nordal) seeds and proanthocyanidin free mutants blocked in four different genes,ant 13, ant 17, ant 18 andant 19. In the wild type barley grown under controlled conditions, maximal rate of synthesis of catechin, procyanidin B3 and procyanidin C2 occurred 8–16 days after flowering. Dihydroquercetin was radioactively labelled by feeding (1-14C)-acetate and (2-14C)-acetate to flowerbuds of a petunia mutant accumulating this flavonoid. When fed to pericarp-testa tissue of wild type barley labelled catechin, procyanidin B3 and procyanidin C2 were synthesized establishing dihydroquercetin as a precursor of these compounds. In addition labelled 2,3-trans-3,4-cis-leucocyanidin was synthesized indicating that this compound is an intermediate. The leucocyanidin was identified by co-chromatography with an authentic standard prepared chemically by reduction of dihydroquercetin with NaBH4. The major product of this reduction, however, was the 2,3-trans-3,4-trans-leucocyanidin. Only mutantant 18-102 accumulated dihydroquercetin in the seeds. Feeding (14C)-dihydroquercetin to pericarp-testa tissue from the mutants revealed thatant 17-139 was capable of synthesizing significant amounts of labelled catechin and procyanidin, whereasant 13-101,ant 13-152,ant 18-102 andant 19-109 synthesized none or only very small amounts of these compounds. It is concluded that (i)ant 18 controls the reduction of dihydroquercetin to 2,3-trans-3,4-cis-leucocyanidin, (ii)ant 19 controls the reduction of the leucocyanidin to catechin, and (iii)ant 13 andant 17 control unidentified steps prior to dihydroquercetin." @default.
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• W2001199176 date "1984-01-01" @default.
• W2001199176 modified "2023-10-14" @default.
• W2001199176 title "Biosynthesis of proanthocyanidins in barley: Genetic control of the conversion of dihydroquercetin to catechin and procyanidins" @default.
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• W2001199176 doi "https://doi.org/10.1007/bf02907552" @default.
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