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• W2001210310 startingPage "6663" @default.
• W2001210310 abstract "It is proposed that the stability of a protein can be increased by selected amino acid substitutions that decrease the configurational entropy of unfolding. Two such substitutions, one of the form Xaa----Pro and the other of the form Gly----Xaa, were constructed in bacteriophage T4 lysozyme at sites consistent with the known three-dimensional structure. Both substitutions stabilize the protein toward reversible and irreversible thermal denaturation at physiological pH. The substitutions have no effect on enzymatic activity. High-resolution crystallographic analysis of the proline-containing mutant protein (Ala-82----Pro) shows that its three-dimensional structure is essentially identical with the wild-type enzyme. The overall structure of the other mutant enzyme (Gly-77----Ala) is also very similar to wild-type lysozyme, although there are localized conformational adjustments in the vicinity of the altered amino acid. The combination of a number of such amino acid replacements, each of which is expected to contribute approximately 1 kcal/mol (1 cal = 4.184 J) to the free energy of folding, may provide a general strategy for substantial improvement in the stability of a protein." @default.
• W2001210310 created "2016-06-24" @default.
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• W2001210310 date "1987-10-01" @default.
• W2001210310 modified "2023-10-14" @default.
• W2001210310 title "Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding." @default.
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• W2001210310 doi "https://doi.org/10.1073/pnas.84.19.6663" @default.
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