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- W2001315494 abstract "The present study was carried out to identify the specific protein kinase C (PKC) isoform involved in regulatory volume decrease (RVD) responses, and to investigate the signal transduction pathways underlying the activation of volume‐sensitive chloride channels in human cervical cancer HT‐3 cells. The role of Ca 2+ in RVD and in the activation of chloride currents was also studied. The time course of RVDs was prolonged by microinjection of PKC‐α antibody but not by PKC‐β or PKC‐γ antibody, and also by exposure to Ca 2+ ‐free medium, in particular when combined with microinjection of EDTA. Immunofluorescence staining showed that hypotonic superfusion evoked the translocation of PKC‐α to the cell membrane, whereas PKC‐β or PKC‐γ remained unaffected. The translocation of PKC‐α was observed a few minutes after hypotonic stress, reaching peak intensity at 30 min, and returned to the cytoplasm 60 min after hypotonic exposure. Western blot analyses showed an increased PKC‐α level in terms of intensity and phosphorylation in the cell membrane, while neither PKC‐β nor PKC‐γ was activated upon hyposmotic challenge. Whole‐cell patch‐clamp studies demonstrated that neomycin and PKC blockers such as staurosporine and H7 inhibited volume‐sensitive chloride currents. The inhibitory effect of neomycin on chloride currents can be reversed by the PKC activator phorbol 12‐myristate, 13‐acetate (PMA). Moreover, the PKC inhibitor and PKC‐α antibody, but not PKC‐β or PKC‐γ antibody, significantly attenuated the chloride currents. The activation of volume‐sensitive chloride currents were insensitive to the changes of intracellular Ca 2+ but required the presence of extracellular Ca 2+ . Our results suggest the involvement of PKC‐α and extracellular Ca 2+ in RVD responses and the activation of volume‐sensitive chloride channels in HT‐3 cells." @default.
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- W2001315494 date "1998-10-01" @default.
- W2001315494 modified "2023-10-15" @default.
- W2001315494 title "Involvement of PKC-α in regulatory volume decrease responses and activation of volume-sensitive chloride channels in human cervical cancer HT-3 cells" @default.
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- W2001315494 doi "https://doi.org/10.1111/j.1469-7793.1998.435be.x" @default.
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