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- W2001330679 abstract "Abstract We have previously demonstrated that macrophages possess an active long chain polyunsaturated fatty acid elongase capable of converting (>85%) gammalinolenic acid (18:3n-6, GLA) to dihomogammalinolenic acid (20:3n-6, DGLA), which, following cell stimulation, is converted to prostaglandin E 1 (PGE 1 ) (Chapkin, R.S. and Coble, K.J. (1991). Biochimica et Biophysica Acta 1085, 365–370). This is noteworthy because PGE 1 is an eicosanoid with anti-aggregatory and anti-inflammatory properties. In the present study, mouse peritoneal macrophages were incubated with [ 14 C]GLA and [ 3 H]-glycerol for 20 hr and subsequently stimulated with calcium ionophore A23187 (phospholipase A 2 activator), phorbol ester (PMA, protein kinase C activator), PMA + A23187, merthiolate (lysophospharide acyltransferase inhibitor) + A23187, or nothing. Following stimulation, PMA + A23187 and merthiolate + A23187 treated cells had significantly ( P 14 C-(PGE 1 ) and [ 14 C]-prostaglandin E 2 (PGE 2 ) biosynthesis compared with A23187, PMA, and nonstimulated treatments. [ 14 C]-fatty acid (primarily DGLA) was primarily incorporated into phosphatidylcholine (PC) (64.8 ± 1.3%, in nonstimulated cells). A23187, PMA, PMA + A23187, and merthiolate + A23187 treatments had significantly ( P 14 C]-PC and increased ( P 3 H]-lyso-PC relative to nonstimulated cells. Therefore, in vitro activation of phospholipase A 2 and inhibition of [ 14 C]DGLA (derived from [ 14 C]GLA) reacylation can significantly ( P 14 C]-(PGE 1 ) biosynthesis. These data indicate the regulatory importance of [ 14 C]DGLA reacylation relative to phospholipase A 2 activity in mouse peritoneal macrophage PGE 1 biosynthesis." @default.
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- W2001330679 date "1993-10-01" @default.
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- W2001330679 title "Phospholipid sources of metabolically elongated gammalinolenic acid: Conversion to prostaglandin E1 in stimulated mouse macrophages" @default.
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- W2001330679 doi "https://doi.org/10.1016/0955-2863(93)90029-v" @default.
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