FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001349198> ?p ?o ?g. }

• W2001349198 abstract "The rapid rate at which cancer cells divide necessitates a mechanism for telomere maintenance, and in approximately 90% of all cancer types the enzyme telomerase is used to maintain the length of telomeric DNA. Telomerase is a multi-subunit enzyme that minimally contains a catalytic protein subunit, hTERT, and an RNA subunit, hTR. Proper assembly of telomerase is critical for its enzymatic activity and therefore is a requirement for the proliferation of most cancer cells. We have developed the first high-throughput screen capable of identifying small molecules that specifically perturb human telomerase assemblage. The screen uses a scintillation proximity assay to identify compounds that prevent a specific and required interaction between hTR and hTERT. Rather than attempting to disrupt all of the individual hTR-hTERT interactions, we focused the screen on the interaction of the CR4-CR5 domain of hTR with hTERT. The screen employs a biotin-labeled derivative of the CR4-CR5 domain of hTR that independently binds [(35)S]hTERT in a functionally relevant manner. The complex between hTERT and biotin-labeled RNA can be captured on streptavidin-coated scintillation proximity beads. Use of 96-well filter plates and a vacuum manifold enables rapid purification of the beads. After optimization, statistical evaluation of the screen generated a Z' factor of 0.6, demonstrating the high precision of the assay." @default.
• W2001349198 created "2016-06-24" @default.
• W2001349198 creator A5034302513 @default.
• W2001349198 creator A5041868084 @default.
• W2001349198 date "2006-06-01" @default.
• W2001349198 modified "2023-09-25" @default.
• W2001349198 title "A high-throughput assay for a human telomerase protein–human telomerase RNA interaction" @default.
• W2001349198 cites W1496772673 @default.
• W2001349198 cites W1497143641 @default.
• W2001349198 cites W1516272650 @default.
• W2001349198 cites W1546807582 @default.
• W2001349198 cites W1908195810 @default.
• W2001349198 cites W1953385895 @default.
• W2001349198 cites W1965743650 @default.
• W2001349198 cites W1977501444 @default.
• W2001349198 cites W1985880165 @default.
• W2001349198 cites W1991001144 @default.
• W2001349198 cites W2000971601 @default.
• W2001349198 cites W2002519680 @default.
• W2001349198 cites W2003842226 @default.
• W2001349198 cites W2004833536 @default.
• W2001349198 cites W2006774455 @default.
• W2001349198 cites W2007613355 @default.
• W2001349198 cites W2018097391 @default.
• W2001349198 cites W2021489383 @default.
• W2001349198 cites W2021525933 @default.
• W2001349198 cites W2021758398 @default.
• W2001349198 cites W2022507419 @default.
• W2001349198 cites W2025811126 @default.
• W2001349198 cites W2026856852 @default.
• W2001349198 cites W2043764151 @default.
• W2001349198 cites W2044283070 @default.
• W2001349198 cites W2048776061 @default.
• W2001349198 cites W2050870921 @default.
• W2001349198 cites W2053620236 @default.
• W2001349198 cites W2058236842 @default.
• W2001349198 cites W2064248337 @default.
• W2001349198 cites W2066058593 @default.
• W2001349198 cites W2073458757 @default.
• W2001349198 cites W2073792387 @default.
• W2001349198 cites W2074134576 @default.
• W2001349198 cites W2079378073 @default.
• W2001349198 cites W2080863990 @default.
• W2001349198 cites W2087160710 @default.
• W2001349198 cites W2098437774 @default.
• W2001349198 cites W2102490688 @default.
• W2001349198 cites W2110419228 @default.
• W2001349198 cites W2115127516 @default.
• W2001349198 cites W2115346781 @default.
• W2001349198 cites W2123111989 @default.
• W2001349198 cites W2124557723 @default.
• W2001349198 cites W2140570233 @default.
• W2001349198 cites W2148013194 @default.
• W2001349198 cites W2148805136 @default.
• W2001349198 cites W2153654013 @default.
• W2001349198 cites W2155043438 @default.
• W2001349198 cites W2167797847 @default.
• W2001349198 cites W3187872748 @default.
• W2001349198 cites W61051146 @default.
• W2001349198 doi "https://doi.org/10.1016/j.ab.2006.03.027" @default.
• W2001349198 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16620757" @default.
• W2001349198 hasPublicationYear "2006" @default.
• W2001349198 type Work @default.
• W2001349198 sameAs 2001349198 @default.
• W2001349198 citedByCount "5" @default.
• W2001349198 countsByYear W20013491982016 @default.
• W2001349198 crossrefType "journal-article" @default.
• W2001349198 hasAuthorship W2001349198A5034302513 @default.
• W2001349198 hasAuthorship W2001349198A5041868084 @default.
• W2001349198 hasConcept C104292427 @default.
• W2001349198 hasConcept C104317684 @default.
• W2001349198 hasConcept C125593758 @default.
• W2001349198 hasConcept C153911025 @default.
• W2001349198 hasConcept C177336024 @default.
• W2001349198 hasConcept C185592680 @default.
• W2001349198 hasConcept C552990157 @default.
• W2001349198 hasConcept C55493867 @default.
• W2001349198 hasConcept C67705224 @default.
• W2001349198 hasConcept C86803240 @default.
• W2001349198 hasConcept C94581717 @default.
• W2001349198 hasConcept C95444343 @default.
• W2001349198 hasConceptScore W2001349198C104292427 @default.
• W2001349198 hasConceptScore W2001349198C104317684 @default.
• W2001349198 hasConceptScore W2001349198C125593758 @default.
• W2001349198 hasConceptScore W2001349198C153911025 @default.
• W2001349198 hasConceptScore W2001349198C177336024 @default.
• W2001349198 hasConceptScore W2001349198C185592680 @default.
• W2001349198 hasConceptScore W2001349198C552990157 @default.
• W2001349198 hasConceptScore W2001349198C55493867 @default.
• W2001349198 hasConceptScore W2001349198C67705224 @default.
• W2001349198 hasConceptScore W2001349198C86803240 @default.
• W2001349198 hasConceptScore W2001349198C94581717 @default.
• W2001349198 hasConceptScore W2001349198C95444343 @default.
• W2001349198 hasLocation W20013491981 @default.
• W2001349198 hasLocation W20013491982 @default.
• W2001349198 hasOpenAccess W2001349198 @default.
• W2001349198 hasPrimaryLocation W20013491981 @default.
• W2001349198 hasRelatedWork W1984279619 @default.
• W2001349198 hasRelatedWork W1990327942 @default.
• W2001349198 hasRelatedWork W1999033684 @default.

• next