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- W2001356168 abstract "The laser flash-induced photoelectric activity of apomembranes or pigment sheets and proteoliposomes associated with a phospholipid impregnated collodion film was measured. Two procedures were used to obtain apomembranes: (i) treatment of purple membranes with hydroxylamine (APOM. 1) and (ii) subsequent treatment of APOM. 1 with sodium borohydride in the dark (APOM. 2). It is shown that the treatment with sodium borohydride decreases the amount of residual bacteriorhodopsin significantly and that APOM. 2 can be used successfully for the regeneration of pigment analogues. However, experiments with one side inhibitor La 3+ ions demonstrate the presence of a heterogeneous orientation of sheets when the association of APOM. 2 with a planar film precedes pigment regeneration. It is concluded that regenerated pigments incorporated into proteoliposomes prior to association with a planar film are the most appropriate system for obtaining accurate measurements of the photoactivity of bacteriorhodopsin analogues." @default.
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- W2001356168 date "1989-10-01" @default.
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- W2001356168 title "Properties of apomembranes treated with sodium borohydride" @default.
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- W2001356168 doi "https://doi.org/10.1016/0022-0728(89)87302-4" @default.
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