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- W2001488045 abstract "The recombinant plasmid pGEM4Z-ras DNA which was methylated on dam and dcm sites outside the Pvull recognition sequence was digested with restriction endonuclease Pvull, and one of the three Pvull sites was about 16-fold less efficient to cleave than either of the other two. On the contrary, the three Pvull sites were cleaved at about the same rate on the unmethylated DNA molecule.The results show that the cleavage inhibition of the methylated DNA on the certain Pvull site was caused by methylation outsidethe Pvull recognition sequence. Maybe a adjacent methylated dam site A was responsible for the less efficient cleavage. This observation suggests that methylation outside the recognition sequence may be considered a new factor in the kinetic experiment of restriction endonuclease." @default.
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- W2001488045 date "1991-01-01" @default.
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- W2001488045 title "The inhibition of restrication endonuclease Pcull cleavage activity by methylaytion outside its recognitionm sequecne" @default.
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- W2001488045 doi "https://doi.org/10.1093/nar/19.20.5703" @default.
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