FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001491651> ?p ?o ?g. }

• W2001491651 endingPage "e17633" @default.
• W2001491651 startingPage "e17633" @default.
• W2001491651 abstract "Bovine spongiform encephalopathy (BSE) surveillance programs have been employed in numerous countries to monitor BSE prevalence and to protect animal and human health. Since 1999, the European Commission (EC) authorized the evaluation and approval of 20 molecular based tests for the rapid detection of the pathological prion protein (PrPsc) in BSE infection. The diagnostic sensitivity, convenience, and speed of these tests have made molecular diagnostics the preferred method for BSE surveillance. The aim of this study was to determine the analytical sensitivity of 4 commercially available BSE rapid-test kits, including the Prionics®-Check WESTERN, the Prionics® Check-PrioSTRIP™, the BioRad® TeSeE™ ELISA, and the IDEXX® HerdChek™ EIA. Performances of these tests were then compared to 2 confirmatory tests, including the BioRad® TeSeE™ Western Blot and the modified Scrapie Associated Fibrils (SAF)/OIE Immunoblot. One 50% w/v homogenate was made from experimentally generated C-type BSE brain tissues in ddH2O. Homogenates were diluted through a background of BSE-negative brainstem homogenate. Masses of both positive and negative tissues in each dilution were calculated to maintain the appropriate tissue amounts for each test platform. Specific concentrated homogenization buffer was added accordingly to maintain the correct buffer condition for each test. ELISA-based tests were evaluated using their respective software/detection platforms. Blot-protocols were evaluated by manual measurements of blot signal density. Detection limitations were determined by fitted curves intersecting the manufacturers' positive/negative criteria. The confirmatory SAF Immunoblot displayed the highest analytical sensitivity, followed by the IDEXX® HerdChek™ EIA, Bio-Rad® TeSeE™ Western Blot, the Bio-Rad® TeSeE™ ELISA, Prionics®-Check PrioSTRIP™, and Prionics®-Check WESTERN™, respectively. Although the tests performed at different levels of sensitivity, the most sensitive and least sensitive of the rapid tests were separated by 2 logs in analytical sensitivity, meeting European performance requirements. All rapid tests appear suitable for targeted BSE surveillance programs, as implemented in Canada." @default.
• W2001491651 created "2016-06-24" @default.
• W2001491651 creator A5012537373 @default.
• W2001491651 creator A5073952768 @default.
• W2001491651 creator A5075810471 @default.
• W2001491651 date "2011-03-11" @default.
• W2001491651 modified "2023-10-16" @default.
• W2001491651 title "A Study on the Analytical Sensitivity of 6 BSE Tests Used by the Canadian BSE Reference Laboratory" @default.
• W2001491651 cites W1636842980 @default.
• W2001491651 cites W1975922727 @default.
• W2001491651 cites W2023529808 @default.
• W2001491651 cites W2024632362 @default.
• W2001491651 cites W2034084051 @default.
• W2001491651 cites W2047225722 @default.
• W2001491651 cites W2048339382 @default.
• W2001491651 cites W2052601863 @default.
• W2001491651 cites W2057570911 @default.
• W2001491651 cites W2059796373 @default.
• W2001491651 cites W2062648430 @default.
• W2001491651 cites W2111584315 @default.
• W2001491651 cites W2121418436 @default.
• W2001491651 cites W2138390885 @default.
• W2001491651 cites W2146086964 @default.
• W2001491651 cites W2155751865 @default.
• W2001491651 cites W2413433335 @default.
• W2001491651 cites W4206684179 @default.
• W2001491651 cites W2041272236 @default.
• W2001491651 doi "https://doi.org/10.1371/journal.pone.0017633" @default.
• W2001491651 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3055877" @default.
• W2001491651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21412419" @default.
• W2001491651 hasPublicationYear "2011" @default.
• W2001491651 type Work @default.
• W2001491651 sameAs 2001491651 @default.
• W2001491651 citedByCount "15" @default.
• W2001491651 countsByYear W20014916512012 @default.
• W2001491651 countsByYear W20014916512013 @default.
• W2001491651 countsByYear W20014916512014 @default.
• W2001491651 countsByYear W20014916512015 @default.
• W2001491651 countsByYear W20014916512016 @default.
• W2001491651 countsByYear W20014916512017 @default.
• W2001491651 countsByYear W20014916512020 @default.
• W2001491651 countsByYear W20014916512021 @default.
• W2001491651 countsByYear W20014916512023 @default.
• W2001491651 crossrefType "journal-article" @default.
• W2001491651 hasAuthorship W2001491651A5012537373 @default.
• W2001491651 hasAuthorship W2001491651A5073952768 @default.
• W2001491651 hasAuthorship W2001491651A5075810471 @default.
• W2001491651 hasBestOaLocation W20014916511 @default.
• W2001491651 hasConcept C104317684 @default.
• W2001491651 hasConcept C105639569 @default.
• W2001491651 hasConcept C142724271 @default.
• W2001491651 hasConcept C144133560 @default.
• W2001491651 hasConcept C159047783 @default.
• W2001491651 hasConcept C2776415932 @default.
• W2001491651 hasConcept C2776735649 @default.
• W2001491651 hasConcept C2779134260 @default.
• W2001491651 hasConcept C2781131914 @default.
• W2001491651 hasConcept C2910001868 @default.
• W2001491651 hasConcept C3019804061 @default.
• W2001491651 hasConcept C3020782553 @default.
• W2001491651 hasConcept C42972112 @default.
• W2001491651 hasConcept C55493867 @default.
• W2001491651 hasConcept C71924100 @default.
• W2001491651 hasConcept C86803240 @default.
• W2001491651 hasConceptScore W2001491651C104317684 @default.
• W2001491651 hasConceptScore W2001491651C105639569 @default.
• W2001491651 hasConceptScore W2001491651C142724271 @default.
• W2001491651 hasConceptScore W2001491651C144133560 @default.
• W2001491651 hasConceptScore W2001491651C159047783 @default.
• W2001491651 hasConceptScore W2001491651C2776415932 @default.
• W2001491651 hasConceptScore W2001491651C2776735649 @default.
• W2001491651 hasConceptScore W2001491651C2779134260 @default.
• W2001491651 hasConceptScore W2001491651C2781131914 @default.
• W2001491651 hasConceptScore W2001491651C2910001868 @default.
• W2001491651 hasConceptScore W2001491651C3019804061 @default.
• W2001491651 hasConceptScore W2001491651C3020782553 @default.
• W2001491651 hasConceptScore W2001491651C42972112 @default.
• W2001491651 hasConceptScore W2001491651C55493867 @default.
• W2001491651 hasConceptScore W2001491651C71924100 @default.
• W2001491651 hasConceptScore W2001491651C86803240 @default.
• W2001491651 hasIssue "3" @default.
• W2001491651 hasLocation W20014916511 @default.
• W2001491651 hasLocation W20014916512 @default.
• W2001491651 hasLocation W20014916513 @default.
• W2001491651 hasLocation W20014916514 @default.
• W2001491651 hasOpenAccess W2001491651 @default.
• W2001491651 hasPrimaryLocation W20014916511 @default.
• W2001491651 hasRelatedWork W1566746840 @default.
• W2001491651 hasRelatedWork W1991237179 @default.
• W2001491651 hasRelatedWork W2016625699 @default.
• W2001491651 hasRelatedWork W2048003269 @default.
• W2001491651 hasRelatedWork W2077455131 @default.
• W2001491651 hasRelatedWork W2098021716 @default.
• W2001491651 hasRelatedWork W2161199496 @default.
• W2001491651 hasRelatedWork W2170686177 @default.
• W2001491651 hasRelatedWork W2409333606 @default.
• W2001491651 hasRelatedWork W3157582652 @default.
• W2001491651 hasVolume "6" @default.

• next