Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001491844> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2001491844 endingPage "411" @default.
- W2001491844 startingPage "402" @default.
- W2001491844 abstract "Abstract The kinetic properties of the soluble adenosine 5′-phosphosulphate sulphohydrolase (APS sulphohydrolase) of bovine liver have been studied. The enzyme exhibits a sharply defined pH optimum (5.4) and is optimally active towards 4.0 mM APS above which concentration inhibition by excess substrate is observed. AMP and inorganic sulphate, the products of the enzymic hydrolysis of APS, inhibit the enzyme non-competitively and competitively, respectively. This pattern of product inhibition is consistent with an ordered uni bi reaction sequence with SO 4 2− as the last-released product. The formation of an intermediate enzyme SO 4 2− complex is implicit in this mechanism. The enzyme is inactivated (irreversibly inhibited) by incubation with ethoxyformic anhydride and by photo-oxidation in the presence of Rose Bengal. The rate of inactivation observed with these reagents is reduced in the presence of ATP, a known competitive inhibitor as APS sulphohydrolase. These results are consistent with the presence of an essential histidine residue at the active site of the enzyme. A possible mechanism of action for APS sulphohydrolase is discussed in the light of these findings." @default.
- W2001491844 created "2016-06-24" @default.
- W2001491844 creator A5006614115 @default.
- W2001491844 creator A5030183796 @default.
- W2001491844 creator A5047127442 @default.
- W2001491844 creator A5057052942 @default.
- W2001491844 date "1973-08-01" @default.
- W2001491844 modified "2023-09-27" @default.
- W2001491844 title "Kinetic properties of the soluble adenosine 5′-phosphosulphate sulphohydrolase from bovine liver" @default.
- W2001491844 cites W1885778179 @default.
- W2001491844 cites W1990881352 @default.
- W2001491844 cites W1992458292 @default.
- W2001491844 cites W2023169505 @default.
- W2001491844 cites W2033412039 @default.
- W2001491844 cites W2038093923 @default.
- W2001491844 cites W2049148583 @default.
- W2001491844 cites W2054979333 @default.
- W2001491844 cites W2060997443 @default.
- W2001491844 cites W2063388095 @default.
- W2001491844 cites W2082352092 @default.
- W2001491844 cites W2335841559 @default.
- W2001491844 cites W2410636739 @default.
- W2001491844 cites W2411016425 @default.
- W2001491844 doi "https://doi.org/10.1016/0005-2744(73)90270-2" @default.
- W2001491844 hasPublicationYear "1973" @default.
- W2001491844 type Work @default.
- W2001491844 sameAs 2001491844 @default.
- W2001491844 citedByCount "12" @default.
- W2001491844 crossrefType "journal-article" @default.
- W2001491844 hasAuthorship W2001491844A5006614115 @default.
- W2001491844 hasAuthorship W2001491844A5030183796 @default.
- W2001491844 hasAuthorship W2001491844A5047127442 @default.
- W2001491844 hasAuthorship W2001491844A5057052942 @default.
- W2001491844 hasConcept C185592680 @default.
- W2001491844 hasConcept C2776991684 @default.
- W2001491844 hasConcept C55493867 @default.
- W2001491844 hasConceptScore W2001491844C185592680 @default.
- W2001491844 hasConceptScore W2001491844C2776991684 @default.
- W2001491844 hasConceptScore W2001491844C55493867 @default.
- W2001491844 hasIssue "2" @default.
- W2001491844 hasLocation W20014918441 @default.
- W2001491844 hasOpenAccess W2001491844 @default.
- W2001491844 hasPrimaryLocation W20014918441 @default.
- W2001491844 hasRelatedWork W1498415432 @default.
- W2001491844 hasRelatedWork W1504841080 @default.
- W2001491844 hasRelatedWork W1593205053 @default.
- W2001491844 hasRelatedWork W1971449617 @default.
- W2001491844 hasRelatedWork W2016119250 @default.
- W2001491844 hasRelatedWork W2016866166 @default.
- W2001491844 hasRelatedWork W2048756163 @default.
- W2001491844 hasRelatedWork W2162798051 @default.
- W2001491844 hasRelatedWork W2314898309 @default.
- W2001491844 hasRelatedWork W2414443002 @default.
- W2001491844 hasVolume "315" @default.
- W2001491844 isParatext "false" @default.
- W2001491844 isRetracted "false" @default.
- W2001491844 magId "2001491844" @default.
- W2001491844 workType "article" @default.