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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001541985> ?p ?o ?g. }

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• W2001541985 abstract "Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) were identified in a collection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)CTGA, GTCA(6N)TGAY and CAC(5N)TGGC, respectively. A methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the adenines that prevent cleavage when methylated (underlined). These results suggest that type I enzymes are abundant in E.coli and many other bacteria, as has been inferred from bacterial genome sequencing projects." @default.
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• W2001541985 date "2005-07-21" @default.
• W2001541985 modified "2023-09-28" @default.
• W2001541985 title "Four new type I restriction enzymes identified in Escherichia coli clinical isolates" @default.
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• W2001541985 doi "https://doi.org/10.1093/nar/gni114" @default.
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