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- W2001557065 abstract "VIsinin-LIke Proteins (VILIPs) are a subfamily of the Neuronal Calcium Sensor (NCS) proteins, which possess both N-myristoylation and EF-hand motifs allowing for a putative 'calcium-myristoyl switch' regulation mechanism. It has previously been established that myristoyl conjugation increases the affinity of proteins for membranes, but, in many cases, a second feature such as a cluster of positively-charged residues is needed for stable membrane binding. The interaction of two members of this family, VILIP-1 and VILIP-3, with Langmuir monolayers as membrane models has been investigated in order to study the effects of both myristoylation and the highly basic region containing conserved poly-lysine residues on membrane association kinetics and binding properties. Results show that in the presence of calcium, N-myristoylation significantly increases the kinetic rate of VILIP adsorption to the membrane. Additionally, the proteins bind to negatively charged phospholipids independently of the conjugated myristate moiety. Besides the regulatory effect of calcium on the rate of binding presumably due to exposure of the myristoyl moiety ascribed to their putative 'calcium-myristoyl switch', VILIP-1 and -3 also engage specific interactions with biomimetic membranes containing phosphatidylinositol 4,5-bisphosphate (PIP2). The presence of PIP2 increases the membrane association rates of both VILIPs. Taken together, these results show the major kinetic role of N-myristoylation for membrane binding, and highlight the critical role of specific phosphoinositide interactions for membrane association of members of the VILIP family." @default.
- W2001557065 created "2016-06-24" @default.
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- W2001557065 date "2014-10-01" @default.
- W2001557065 modified "2023-09-29" @default.
- W2001557065 title "Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins" @default.
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- W2001557065 cites W1541663996 @default.
- W2001557065 cites W1670420367 @default.
- W2001557065 cites W1849374155 @default.
- W2001557065 cites W1966998035 @default.
- W2001557065 cites W1968797683 @default.
- W2001557065 cites W1975038690 @default.
- W2001557065 cites W1976573258 @default.
- W2001557065 cites W1977493393 @default.
- W2001557065 cites W1978287464 @default.
- W2001557065 cites W1982722263 @default.
- W2001557065 cites W1985764146 @default.
- W2001557065 cites W1986698046 @default.
- W2001557065 cites W1988640416 @default.
- W2001557065 cites W1990524685 @default.
- W2001557065 cites W1991709761 @default.
- W2001557065 cites W1999554227 @default.
- W2001557065 cites W2001473402 @default.
- W2001557065 cites W2007513923 @default.
- W2001557065 cites W2008264122 @default.
- W2001557065 cites W2009170466 @default.
- W2001557065 cites W2012175414 @default.
- W2001557065 cites W2014687101 @default.
- W2001557065 cites W2015791803 @default.
- W2001557065 cites W2021530966 @default.
- W2001557065 cites W2025572688 @default.
- W2001557065 cites W2028219488 @default.
- W2001557065 cites W2036014270 @default.
- W2001557065 cites W2036146475 @default.
- W2001557065 cites W2036258428 @default.
- W2001557065 cites W2038078222 @default.
- W2001557065 cites W2042269004 @default.
- W2001557065 cites W2042292475 @default.
- W2001557065 cites W2043175613 @default.
- W2001557065 cites W2048790396 @default.
- W2001557065 cites W2048929195 @default.
- W2001557065 cites W2054799935 @default.
- W2001557065 cites W2061775710 @default.
- W2001557065 cites W2061934035 @default.
- W2001557065 cites W2062431951 @default.
- W2001557065 cites W2062574001 @default.
- W2001557065 cites W2073154248 @default.
- W2001557065 cites W2076838810 @default.
- W2001557065 cites W2081230748 @default.
- W2001557065 cites W2085152901 @default.
- W2001557065 cites W2085700736 @default.
- W2001557065 cites W2087464785 @default.
- W2001557065 cites W2088997949 @default.
- W2001557065 cites W2094572278 @default.
- W2001557065 cites W2095390966 @default.
- W2001557065 cites W2095490475 @default.
- W2001557065 cites W2112986837 @default.
- W2001557065 cites W2113456839 @default.
- W2001557065 cites W2115303430 @default.
- W2001557065 cites W2128894233 @default.
- W2001557065 cites W2133066848 @default.
- W2001557065 cites W2134283668 @default.
- W2001557065 cites W2134701313 @default.
- W2001557065 cites W2154775170 @default.
- W2001557065 cites W2161480259 @default.
- W2001557065 cites W2167709066 @default.
- W2001557065 cites W2168384429 @default.
- W2001557065 cites W2315288016 @default.
- W2001557065 cites W2320128071 @default.
- W2001557065 cites W2329814556 @default.
- W2001557065 cites W2333133916 @default.
- W2001557065 doi "https://doi.org/10.1016/j.bbamem.2014.06.021" @default.
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