FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001567457> ?p ?o ?g. }

• W2001567457 endingPage "e104141" @default.
• W2001567457 startingPage "e104141" @default.
• W2001567457 abstract "In the yeast Saccharomyces cerevisiae two alcohol acetyltransferases (AATases), Atf1 and Atf2, condense short chain alcohols with acetyl-CoA to produce volatile acetate esters. Such esters are, in large part, responsible for the distinctive flavors and aromas of fermented beverages including beer, wine, and sake. Atf1 and Atf2 localize to the endoplasmic reticulum (ER) and Atf1 is known to localize to lipid droplets (LDs). The mechanism and function of these localizations are unknown. Here, we investigate potential mechanisms of Atf1 and Atf2 membrane association. Segments of the N- and C-terminal domains of Atf1 (residues 24-41 and 508-525, respectively) are predicted to be amphipathic helices. Truncations of these helices revealed that the terminal domains are essential for ER and LD association. Moreover, mutations of the basic or hydrophobic residues in the N-terminal helix and hydrophobic residues in the C-terminal helix disrupted ER association and subsequent sorting from the ER to LDs. Similar amphipathic helices are found at both ends of Atf2, enabling ER and LD association. As was the case with Atf1, mutations to the N- and C-terminal helices of Atf2 prevented membrane association. Sequence comparison of the AATases from Saccharomyces, non-Saccharomyces yeast (K. lactis and P. anomala) and fruits species (C. melo and S. lycopersicum) showed that only AATases from Saccharomyces evolved terminal amphipathic helices. Heterologous expression of these orthologs in S. cerevisiae revealed that the absence of terminal amphipathic helices eliminates LD association. Combined, the results of this study suggest a common mechanism of membrane association for AATases via dual N- and C-terminal amphipathic helices." @default.
• W2001567457 created "2016-06-24" @default.
• W2001567457 creator A5041649271 @default.
• W2001567457 creator A5051470799 @default.
• W2001567457 date "2014-08-05" @default.
• W2001567457 modified "2023-09-26" @default.
• W2001567457 title "Dual N- and C-Terminal Helices Are Required for Endoplasmic Reticulum and Lipid Droplet Association of Alcohol Acetyltransferases in Saccharomyces cerevisiae" @default.
• W2001567457 cites W149265968 @default.
• W2001567457 cites W1505282200 @default.
• W2001567457 cites W1587494622 @default.
• W2001567457 cites W1726545745 @default.
• W2001567457 cites W1914512945 @default.
• W2001567457 cites W1965519753 @default.
• W2001567457 cites W1966360455 @default.
• W2001567457 cites W1971612445 @default.
• W2001567457 cites W1971804899 @default.
• W2001567457 cites W1974719543 @default.
• W2001567457 cites W1979112749 @default.
• W2001567457 cites W1983486856 @default.
• W2001567457 cites W1984198012 @default.
• W2001567457 cites W1990601606 @default.
• W2001567457 cites W1993033605 @default.
• W2001567457 cites W1996355353 @default.
• W2001567457 cites W1997238838 @default.
• W2001567457 cites W2006385755 @default.
• W2001567457 cites W2011805571 @default.
• W2001567457 cites W2015666741 @default.
• W2001567457 cites W2026559278 @default.
• W2001567457 cites W2037141003 @default.
• W2001567457 cites W2040632126 @default.
• W2001567457 cites W2040694939 @default.
• W2001567457 cites W2044073767 @default.
• W2001567457 cites W2046393457 @default.
• W2001567457 cites W2065926860 @default.
• W2001567457 cites W2066660445 @default.
• W2001567457 cites W2073413181 @default.
• W2001567457 cites W2073900832 @default.
• W2001567457 cites W2080835548 @default.
• W2001567457 cites W2081906422 @default.
• W2001567457 cites W2082537227 @default.
• W2001567457 cites W2082573660 @default.
• W2001567457 cites W2089858560 @default.
• W2001567457 cites W2090294224 @default.
• W2001567457 cites W2105383906 @default.
• W2001567457 cites W2105765079 @default.
• W2001567457 cites W2106882534 @default.
• W2001567457 cites W2110573326 @default.
• W2001567457 cites W2117788306 @default.
• W2001567457 cites W2119993590 @default.
• W2001567457 cites W2120811155 @default.
• W2001567457 cites W2121233961 @default.
• W2001567457 cites W2126273331 @default.
• W2001567457 cites W2132632499 @default.
• W2001567457 cites W2135826679 @default.
• W2001567457 cites W2136364313 @default.
• W2001567457 cites W2151363300 @default.
• W2001567457 cites W2151442352 @default.
• W2001567457 cites W2152770371 @default.
• W2001567457 cites W2152920085 @default.
• W2001567457 cites W2154046032 @default.
• W2001567457 cites W2155387553 @default.
• W2001567457 cites W2169805130 @default.
• W2001567457 cites W2171208384 @default.
• W2001567457 doi "https://doi.org/10.1371/journal.pone.0104141" @default.
• W2001567457 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4122449" @default.
• W2001567457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25093817" @default.
• W2001567457 hasPublicationYear "2014" @default.
• W2001567457 type Work @default.
• W2001567457 sameAs 2001567457 @default.
• W2001567457 citedByCount "21" @default.
• W2001567457 countsByYear W20015674572015 @default.
• W2001567457 countsByYear W20015674572016 @default.
• W2001567457 countsByYear W20015674572017 @default.
• W2001567457 countsByYear W20015674572018 @default.
• W2001567457 countsByYear W20015674572019 @default.
• W2001567457 countsByYear W20015674572020 @default.
• W2001567457 countsByYear W20015674572021 @default.
• W2001567457 countsByYear W20015674572022 @default.
• W2001567457 countsByYear W20015674572023 @default.
• W2001567457 crossrefType "journal-article" @default.
• W2001567457 hasAuthorship W2001567457A5041649271 @default.
• W2001567457 hasAuthorship W2001567457A5051470799 @default.
• W2001567457 hasBestOaLocation W20015674571 @default.
• W2001567457 hasConcept C158617107 @default.
• W2001567457 hasConcept C185592680 @default.
• W2001567457 hasConcept C2777576037 @default.
• W2001567457 hasConcept C2779222958 @default.
• W2001567457 hasConcept C55493867 @default.
• W2001567457 hasConcept C86803240 @default.
• W2001567457 hasConcept C95444343 @default.
• W2001567457 hasConceptScore W2001567457C158617107 @default.
• W2001567457 hasConceptScore W2001567457C185592680 @default.
• W2001567457 hasConceptScore W2001567457C2777576037 @default.
• W2001567457 hasConceptScore W2001567457C2779222958 @default.
• W2001567457 hasConceptScore W2001567457C55493867 @default.
• W2001567457 hasConceptScore W2001567457C86803240 @default.
• W2001567457 hasConceptScore W2001567457C95444343 @default.
• W2001567457 hasIssue "8" @default.

• next