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• W2001569101 endingPage "1901" @default.
• W2001569101 startingPage "1887" @default.
• W2001569101 abstract "Almost a decade has passed since the first paper describing the isolation and maintenance of precision-cut liver slices produced using a mechanical tissue slicer was published (1). Although tissue slices of various organs have been employed as an in vitro system for several decades, the lack of reproducibility within the slices and the relatively limited viability of the tissue preparations has prevented a widespread acceptance of the technique. The production of an automated slicer, capable of reproducibly producing relatively thin slices of tissue, as well as the development of a dynamic organ culture system, overcame several of these obstacles. Since that time, significant advances in the methods to produce and culture tissue slices have been made, as well as the application of the technique to several other organs, including kidney, lung and heart. This review will i) summarize the historical use of tissue slices prior to the development of the precision-cut tissue slice system; ii) briefly analyze current methods to produce precision-cut liver, kidney, lung and heart slices; and iii) discuss the applications of this powerful in vitro system to the disciplines of pharmacology and toxicology." @default.
• W2001569101 created "2016-06-24" @default.
• W2001569101 creator A5019067799 @default.
• W2001569101 creator A5024842461 @default.
• W2001569101 creator A5028679979 @default.
• W2001569101 date "1995-10-01" @default.
• W2001569101 modified "2023-10-17" @default.
• W2001569101 title "Precision-cut tissue slices: Applications in pharmacology and toxicology" @default.
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