Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001582223> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2001582223 endingPage "S231" @default.
- W2001582223 startingPage "S231" @default.
- W2001582223 abstract "Mitochondrial Ca2+ sequestration is a well-known process that is involved in various physiological and pathological mechanisms. Using isolated suspended mitochondria one unique mitochondrial Ca2+ uniporter was considered to account ubiquitously for the transfer of Ca2+ into these organelles. However, by applying alternative techniques for measuring mitochondrial Ca2+ uptake evidences for molecularly distinct mitochondrial Ca2+ carriers accumulated recently. Herein we compared different methodical approaches of studying mitochondrial Ca2+ uptake. Patch clamp technique on mitoplasts from endothelial and HeLa cells revealed the existence of three and two mitoplast Ca2+ currents (ICaMito), respectively. According to their conductance, these channels were named small (s-), intermediate (i-), large (l-) and extra-large (xl-) mitoplast Ca2+ currents (MCC). i-MCC was found in mitoplasts of both cell types whereas s-MCC and l-MCC or xl-MCC were/was exclusively found in mitoplasts from endothelial cells or HeLa cells. The comparison of mitochondrial Ca2+ signals, measured either indirectly by sensing extra-mitochondrial Ca2+ or directly by recording changes of the matrix Ca2+, showed different Ca2+ sensitivities of the distinct mitochondrial Ca2+ uptake routes. Subpopulations of mitochondria with different Ca2+ uptake capacities in intact endothelial cells could be identified using Rhod-2/AM. In contrast, cells expressing mitochondrial targeted pericam or cameleon (4mtD3cpv) showed homogeneous mitochondrial Ca2+ signals in response to cell stimulation. The comparison of different experimental approaches and protocols using isolated organelles, permeabilized and intact cells, pointed to cell-type specific and versatile pathways for mitochondrial Ca2+ uptake. Moreover, this work highlights the necessity of the utilization of multiple technical approaches to study the complexity of mitochondrial Ca2+ homeostasis." @default.
- W2001582223 created "2016-06-24" @default.
- W2001582223 creator A5004970577 @default.
- W2001582223 creator A5010210218 @default.
- W2001582223 creator A5013592342 @default.
- W2001582223 creator A5061821707 @default.
- W2001582223 creator A5065415875 @default.
- W2001582223 date "2009-01-01" @default.
- W2001582223 modified "2023-10-16" @default.
- W2001582223 title "Successful differentiation of SCN2.2 cells which stably express Yellow Cameleon 3.6" @default.
- W2001582223 doi "https://doi.org/10.1016/j.neures.2009.09.1299" @default.
- W2001582223 hasPublicationYear "2009" @default.
- W2001582223 type Work @default.
- W2001582223 sameAs 2001582223 @default.
- W2001582223 citedByCount "0" @default.
- W2001582223 crossrefType "journal-article" @default.
- W2001582223 hasAuthorship W2001582223A5004970577 @default.
- W2001582223 hasAuthorship W2001582223A5010210218 @default.
- W2001582223 hasAuthorship W2001582223A5013592342 @default.
- W2001582223 hasAuthorship W2001582223A5061821707 @default.
- W2001582223 hasAuthorship W2001582223A5065415875 @default.
- W2001582223 hasConcept C11701378 @default.
- W2001582223 hasConcept C12554922 @default.
- W2001582223 hasConcept C1491633281 @default.
- W2001582223 hasConcept C168240541 @default.
- W2001582223 hasConcept C173358068 @default.
- W2001582223 hasConcept C181199279 @default.
- W2001582223 hasConcept C181911157 @default.
- W2001582223 hasConcept C185592680 @default.
- W2001582223 hasConcept C2777366897 @default.
- W2001582223 hasConcept C28859421 @default.
- W2001582223 hasConcept C35341161 @default.
- W2001582223 hasConcept C55493867 @default.
- W2001582223 hasConcept C75385678 @default.
- W2001582223 hasConcept C86803240 @default.
- W2001582223 hasConcept C95444343 @default.
- W2001582223 hasConcept C98539663 @default.
- W2001582223 hasConceptScore W2001582223C11701378 @default.
- W2001582223 hasConceptScore W2001582223C12554922 @default.
- W2001582223 hasConceptScore W2001582223C1491633281 @default.
- W2001582223 hasConceptScore W2001582223C168240541 @default.
- W2001582223 hasConceptScore W2001582223C173358068 @default.
- W2001582223 hasConceptScore W2001582223C181199279 @default.
- W2001582223 hasConceptScore W2001582223C181911157 @default.
- W2001582223 hasConceptScore W2001582223C185592680 @default.
- W2001582223 hasConceptScore W2001582223C2777366897 @default.
- W2001582223 hasConceptScore W2001582223C28859421 @default.
- W2001582223 hasConceptScore W2001582223C35341161 @default.
- W2001582223 hasConceptScore W2001582223C55493867 @default.
- W2001582223 hasConceptScore W2001582223C75385678 @default.
- W2001582223 hasConceptScore W2001582223C86803240 @default.
- W2001582223 hasConceptScore W2001582223C95444343 @default.
- W2001582223 hasConceptScore W2001582223C98539663 @default.
- W2001582223 hasLocation W20015822231 @default.
- W2001582223 hasOpenAccess W2001582223 @default.
- W2001582223 hasPrimaryLocation W20015822231 @default.
- W2001582223 hasRelatedWork W1981642705 @default.
- W2001582223 hasRelatedWork W2018776448 @default.
- W2001582223 hasRelatedWork W2153923384 @default.
- W2001582223 hasRelatedWork W2337885846 @default.
- W2001582223 hasRelatedWork W2415549374 @default.
- W2001582223 hasRelatedWork W2467413104 @default.
- W2001582223 hasRelatedWork W3097428578 @default.
- W2001582223 hasRelatedWork W3109874725 @default.
- W2001582223 hasRelatedWork W3112581955 @default.
- W2001582223 hasRelatedWork W3164241571 @default.
- W2001582223 hasVolume "65" @default.
- W2001582223 isParatext "false" @default.
- W2001582223 isRetracted "false" @default.
- W2001582223 magId "2001582223" @default.
- W2001582223 workType "article" @default.