Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001631639> ?p ?o ?g. }
- W2001631639 endingPage "401" @default.
- W2001631639 startingPage "394" @default.
- W2001631639 abstract "The mechanism of action of the heat-stable enterotoxin STa secreted from enterotoxigenic forms of Escherichia coli has remained elusive, in part due to a tedious, low-yield purification procedure. We report here a method for obtaining large amounts of a biologically active lysine-containing analog of STa. Initial attempts to express the toxin using an expression vector that did not encode a signal sequence resulted in no biologically active material being recovered either from lysed cells or as a secretory product. However, use of the secretion vector pJAL36, which contains the STII enterotoxin signal sequence, allowed large amounts of an STa derivative containing the additional sequence Ser-Thr-Lys at the amino terminus of the mature enterotoxin to be readily purified from culture supernatants. This enterotoxin analog, known as KSTa-1, was equal in biological and receptor binding activity to the native toxin STa. The lysine residue present in KSTa-1 promises to be useful as a reactive amino acid that is readily derivatized to allow coupling of the enterotoxin to supports for affinity chromatography and antigenic conjugates. Additionally, the insertion of the lysine residue carboxy terminal to the Ser-Thr sequence adds a reversible “handle” to the toxin sequence in that the Ser-Thr-Lys segment can be removed by treatment with trypsin, releasing the native form of STa." @default.
- W2001631639 created "2016-06-24" @default.
- W2001631639 creator A5025045987 @default.
- W2001631639 creator A5027098806 @default.
- W2001631639 creator A5032773579 @default.
- W2001631639 creator A5058144983 @default.
- W2001631639 date "1991-10-01" @default.
- W2001631639 modified "2023-09-26" @default.
- W2001631639 title "High-level expression and secretion of a lysine-containing analog of Escherichia coli heat-stable enterotoxin'" @default.
- W2001631639 cites W1545964569 @default.
- W2001631639 cites W1561793264 @default.
- W2001631639 cites W171398818 @default.
- W2001631639 cites W1762178374 @default.
- W2001631639 cites W1904526602 @default.
- W2001631639 cites W1929143943 @default.
- W2001631639 cites W1930393440 @default.
- W2001631639 cites W1933189170 @default.
- W2001631639 cites W1962258665 @default.
- W2001631639 cites W1965409746 @default.
- W2001631639 cites W1982486162 @default.
- W2001631639 cites W1999862146 @default.
- W2001631639 cites W2020963889 @default.
- W2001631639 cites W2038461827 @default.
- W2001631639 cites W2049105193 @default.
- W2001631639 cites W2053278469 @default.
- W2001631639 cites W2061721999 @default.
- W2001631639 cites W2065729339 @default.
- W2001631639 cites W2067237259 @default.
- W2001631639 cites W2082686208 @default.
- W2001631639 cites W2085620013 @default.
- W2001631639 cites W2087453819 @default.
- W2001631639 cites W2095353164 @default.
- W2001631639 cites W2107182550 @default.
- W2001631639 cites W2126652357 @default.
- W2001631639 cites W2148784430 @default.
- W2001631639 cites W2152134577 @default.
- W2001631639 cites W2163318604 @default.
- W2001631639 cites W2245536552 @default.
- W2001631639 cites W2398828725 @default.
- W2001631639 cites W2470854422 @default.
- W2001631639 doi "https://doi.org/10.1016/1046-5928(91)90100-w" @default.
- W2001631639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1821815" @default.
- W2001631639 hasPublicationYear "1991" @default.
- W2001631639 type Work @default.
- W2001631639 sameAs 2001631639 @default.
- W2001631639 citedByCount "4" @default.
- W2001631639 countsByYear W20016316392013 @default.
- W2001631639 crossrefType "journal-article" @default.
- W2001631639 hasAuthorship W2001631639A5025045987 @default.
- W2001631639 hasAuthorship W2001631639A5027098806 @default.
- W2001631639 hasAuthorship W2001631639A5032773579 @default.
- W2001631639 hasAuthorship W2001631639A5058144983 @default.
- W2001631639 hasConcept C104317684 @default.
- W2001631639 hasConcept C10858879 @default.
- W2001631639 hasConcept C167625842 @default.
- W2001631639 hasConcept C185592680 @default.
- W2001631639 hasConcept C2776016237 @default.
- W2001631639 hasConcept C2777367657 @default.
- W2001631639 hasConcept C2777871010 @default.
- W2001631639 hasConcept C49039625 @default.
- W2001631639 hasConcept C515207424 @default.
- W2001631639 hasConcept C547475151 @default.
- W2001631639 hasConcept C55493867 @default.
- W2001631639 hasConcept C66999527 @default.
- W2001631639 hasConcept C86803240 @default.
- W2001631639 hasConcept C96178990 @default.
- W2001631639 hasConceptScore W2001631639C104317684 @default.
- W2001631639 hasConceptScore W2001631639C10858879 @default.
- W2001631639 hasConceptScore W2001631639C167625842 @default.
- W2001631639 hasConceptScore W2001631639C185592680 @default.
- W2001631639 hasConceptScore W2001631639C2776016237 @default.
- W2001631639 hasConceptScore W2001631639C2777367657 @default.
- W2001631639 hasConceptScore W2001631639C2777871010 @default.
- W2001631639 hasConceptScore W2001631639C49039625 @default.
- W2001631639 hasConceptScore W2001631639C515207424 @default.
- W2001631639 hasConceptScore W2001631639C547475151 @default.
- W2001631639 hasConceptScore W2001631639C55493867 @default.
- W2001631639 hasConceptScore W2001631639C66999527 @default.
- W2001631639 hasConceptScore W2001631639C86803240 @default.
- W2001631639 hasConceptScore W2001631639C96178990 @default.
- W2001631639 hasIssue "5-6" @default.
- W2001631639 hasLocation W20016316391 @default.
- W2001631639 hasLocation W20016316392 @default.
- W2001631639 hasOpenAccess W2001631639 @default.
- W2001631639 hasPrimaryLocation W20016316391 @default.
- W2001631639 hasRelatedWork W1246780373 @default.
- W2001631639 hasRelatedWork W1516175917 @default.
- W2001631639 hasRelatedWork W1637080987 @default.
- W2001631639 hasRelatedWork W1685063743 @default.
- W2001631639 hasRelatedWork W1822070686 @default.
- W2001631639 hasRelatedWork W1833270290 @default.
- W2001631639 hasRelatedWork W1961746711 @default.
- W2001631639 hasRelatedWork W2038754224 @default.
- W2001631639 hasRelatedWork W2153095588 @default.
- W2001631639 hasRelatedWork W2416608750 @default.
- W2001631639 hasVolume "2" @default.
- W2001631639 isParatext "false" @default.
- W2001631639 isRetracted "false" @default.